Role of NKp46 Expression in Cytokine Production by CD56-Positive NK Cells in the Peripheral Blood and the Uterine Endometrium
Article first published online: 11 JAN 2013
© 2013 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 69, Issue 3, pages 202–211, March 2013
How to Cite
Role of NKp46 Expression in Cytokine Production by CD56-Positive NK Cells in the Peripheral Blood and the Uterine Endometrium. Am J Reprod Immunol 2013; 69: 202–211, , , , , , , , .
- Issue published online: 6 FEB 2013
- Article first published online: 11 JAN 2013
- Manuscript Accepted: 19 NOV 2012
- Manuscript Received: 26 AUG 2012
- natural cytotoxicity receptor;
- natural killer cell;
To investigate the role of natural cytotoxicity receptor, NKp46 expression in cytokine-producing NK cells.
Method of Study
CD56+/NKp46+ NK cells from the peripheral blood and the uterine endometrium were magnetically separated. IFN-γ, TNF-α, IL-4, IL-10, and TGF-β1 expressions on NK cells were investigated using multicolor flow cytometry.
Peripheral blood and uterine endometrial NK cells were grouped into 4 subpopulations based upon the degree of CD56 and NKp46 expressions. NKp46 expression was associated with higher frequency of cytokine-producing NK cells, including CD56dimNK cells. The percentage of TNF-α+ and IL-10+ NK cells per total CD56+/NKp46+ NK cells in the uterine endometrium showed a significant correlation with those of the peripheral blood in all subpopulations, but that of IFN-γ+, IL-4+, and TGF-β1+ NK cells showed partial correlation.
Expression of NKp46 is involved in cytokine production of CD56+ NK cells in the peripheral blood and the uterine endometrium.