B7 Costimulation and Intracellular Indoleamine-2,3-Dioxygenase Expression in Peripheral Blood of Healthy Pregnant and Pre-Eclamptic Women
Article first published online: 7 JAN 2013
© 2013 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 69, Issue 3, pages 264–271, March 2013
How to Cite
B7 costimulation and intracellular indoleamine-2,3-dioxygenase expression in peripheral blood of healthy pregnant and pre-eclamptic women. Am J Reprod Immunol 2013; 69: 264–271, , , , , .
- Issue published online: 6 FEB 2013
- Article first published online: 7 JAN 2013
- Manuscript Accepted: 5 DEC 2012
- Manuscript Received: 31 JUL 2012
- indoleamine-2,3-dioxygenase, monocyte;
- T cell
We determined the frequency of activated (CD11b+) monocytes expressing B7-1, B7-2, B7-H1, and B-7H2, and that of T cells and T helper cells expressing CD28, CTLA-4, PD-1, and ICOS in peripheral blood samples from normal pregnant (NP) and pre-eclamptic (PE) women. We also examined the intracellular expression of indoleamine-2,3-dioxygenase (IDO).
Method of study
We measured the expression of the above markers using flow-cytometry in peripheral blood samples from 20 NP and 20 PE women in the third trimester.
The frequency of B7-1 and B7-2 expressing activated monocytes and that of IDO expressing T-lymphocytes was lower in PE than in NP.
Lower expression of B7-1 and B7-2 proteins on peripheral monocytes in PE might indicate a secondary regulatory mechanism in response to the ongoing systemic maternal inflammation. IDO plays an important role in the pregnancy-specific immune tolerance, and might be a contributing factor in the pathogenesis of PE.