Evidence for a Role for the Adaptive Immune Response in Human Term Parturition

Authors

  • Nardhy Gomez-Lopez,

    Corresponding author
    1. Department of Obstetrics and Gynecology, School of Medicine, Wayne State University, Detroit, MI, USA
    2. Perinatology Research Branch, NICHD, NIH, DHHS, Bethesda, MD, USA
    • Research Direction and Department of Nutrition Research, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
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  • Rodrigo Vega-Sanchez,

    1. Research Direction and Department of Nutrition Research, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
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  • Marisol Castillo-Castrejon,

    1. Unidad de Vinculación de la Facultad de Medicina, UNAM en el Instituto Nacional de Medicina Genómica
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  • Roberto Romero,

    1. Perinatology Research Branch, NICHD, NIH, DHHS, Bethesda, MD, USA
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  • Karen Cubeiro-Arreola,

    1. Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico
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  • Felipe Vadillo-Ortega

    Corresponding author
    1. Unidad de Vinculación de la Facultad de Medicina, UNAM en el Instituto Nacional de Medicina Genómica
    • Research Direction and Department of Nutrition Research, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
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Correspondence

Felipe Vadillo-Ortega, Biochemistry Department, Facultad de Medicina, UNAM. Av. Universidad 3000, Ciudad Universitaria, Torre de Investigación 3er piso, Mexico City, Zip 04510, Mexico.

E-mail: felipe.vadillo@gmail.com

and

Nardhy Gomez-Lopez, Department of Obstetrics and Gynecology, Perinatology Research Branch, Wayne State University, Detroit, MI 48201, USA.

E-mail: nardhy.gomez-lopez@wayne.edu

Abstract

Problem

Spontaneous labor at term involves leukocyte recruitment and infiltration into the choriodecidua; yet, characterization of these leukocytes and their immunological mediators is incomplete. The purpose of this study was to characterize the immunophenotype of choriodecidual leukocytes as well as the expression of inflammatory mediators in human spontaneous parturition at term.

Method of study

Choriodecidual leukocytes were analyzed by FACS, immunohistochemistry, and RT-PCR in three different groups: (i) preterm gestation delivered for medical indications without labor; (ii) term pregnancy without labor; and (iii) term pregnancy after spontaneous labor.

Results

Two T-cell subsets of memory-like T cells (CD3+CD4+CD45RO+ and CD3+CD4CD8CD45RO+ cells) were identified in the choriodecidua of women who had spontaneous labor. Evidence for an extensive immune signaling network composed of chemokines (CXCL8 and CXCL10), chemokine receptors (CXCR1-3), cytokines (IL-1β and TNF-α), cell adhesion molecules, and MMP-9 was identified in these cells during spontaneous labor at term.

Conclusions

The influx of memory-like T cells in the choriodecidua and the evidence that they are active by producing chemokines and cytokines, and expressing chemokine receptors, cell adhesion molecules, and a matrix-degrading enzyme provides support for the participation of the adaptive immune system in the mechanisms of spontaneous parturition at term.

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