Altered Transcriptional Regulation of Cytokines, Growth Factors, and Apoptotic Proteins in the Endometrium of Infertile Women with Chronic Endometritis

Authors

  • Cinzia Di Pietro,

    Corresponding author
    • Dipartimento G.F. Ingrassia, Biologia Generale, Biologia Cellulare, Genetica Molecolare G Sichel, Az. Policlinico, Università di Catania, Catania, Italy
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  • Ettore Cicinelli,

    1. Dipartimento di Ginecologia Ostetricia e Neonatologia, Università di Bari, Bari, Italy
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  • Maria R. Guglielmino,

    1. Dipartimento G.F. Ingrassia, Biologia Generale, Biologia Cellulare, Genetica Molecolare G Sichel, Az. Policlinico, Università di Catania, Catania, Italy
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  • Marco Ragusa,

    1. Dipartimento G.F. Ingrassia, Biologia Generale, Biologia Cellulare, Genetica Molecolare G Sichel, Az. Policlinico, Università di Catania, Catania, Italy
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  • Marco Farina,

    1. Dipartimento di Specialità Medico-Chirurgiche, Clinica Ostetrica e Ginecologica, Az. Ospedaliero-Universitaria “Policlinico V. Emanuele”, Università di Catania, Catania, Italy
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  • Marco A. Palumbo,

    1. Dipartimento di Specialità Medico-Chirurgiche, Clinica Ostetrica e Ginecologica, Az. Ospedaliero-Universitaria “Policlinico V. Emanuele”, Università di Catania, Catania, Italy
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  • Antonio Cianci

    1. Dipartimento di Specialità Medico-Chirurgiche, Clinica Ostetrica e Ginecologica, Az. Ospedaliero-Universitaria “Policlinico V. Emanuele”, Università di Catania, Catania, Italy
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Correspondence

Cinzia Di Pietro, Dipartimento G.F. Ingrassia, Biologia Generale, Biologia Cellulare, Genetica Molecolare G Sichel, Az. Policlinico, Università di Catania, Via S.Sofia 87, 95123 Catania, Italy.

E-mail: dipietro@unict.it

Abstract

Problem

Chronic endometritis (CE) is a poorly investigated and probably underestimated pathology, which may cause abnormal uterine bleeding (AUB), pain, and reproductive failures. Due to undefined symptoms and the normal presence of leukocytes in the endometrial mucosa, diagnosis may be missed. Fluid hysteroscopy is a reliable technique for diagnosing this pathology. Few data exist on the biochemical and paracrine alterations that occur in the endometrium of women diagnosed with CE. The aim of the study was to find molecular modification in endometrium related to CE.

Method of study

Sixteen women with hysteroscopic and histological diagnosis of CE and 10 healthy women as controls were enrolled. We compared the endometrial expression profile of 25 genes encoding proteins involved in the inflammatory response, proliferation, and apoptosis in endometrium during implantation window, using high-throughput real-time RT-PCR.

Results

In women with CE, the endometrial expression of some genes was significantly altered. In particular, IGFBP1, BCL2, and BAX were up-regulated, while IL11, CCL4, IGF1, and CASP8 were down-regulated.

Conclusion

The altered gene endometrial expression may explain the impaired endometrial receptivity and the finding of endometrial hyperplastic lesions in women affected by CE.

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