Cyclic Stretch Augments Production of Neutrophil Chemokines and Matrix Metalloproteinases-1 (MMP-1) from Human Decidual Cells, and the Production was Reduced by Progesterone
Article first published online: 19 FEB 2013
© 2013 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 69, Issue 5, pages 454–462, May 2013
How to Cite
Cyclic stretch augments production of neutrophil chemokines and matrix metalloproteinases-1 (MMP-1) from human decidual Cells, and the production was reduced by progesterone. Am J Reprod Immunol 2013; 69: 454–462., , , , , , , , , , , .
- Issue published online: 12 APR 2013
- Article first published online: 19 FEB 2013
- Manuscript Accepted: 15 JAN 2013
- Manuscript Received: 15 NOV 2012
- Ministry of Health, Labor and Welfare
- Ministry of Education, Culture, Sports, Science and Technology
- Japan–China Medical Association
- Kanzawa Medical Research Foundation
- Growth-regulated oncogene alpha (GROα);
- interleukin-8 (IL-8);
- preterm labor;
- progesterone (P4)
The purpose of this study was to evaluate the impact of mechanical stretch caused by uterine contraction and progesterone (P4) on decidual cells (DC), neutrophil chemokines, and MMP-1 expression.
Method of study
DC were cultured, and cyclic stretch was applied using a computer-operated cell stretch system. Interleukin (IL)-8, growth-regulated oncogene (GRO) α, matrix metalloproteinase (MMP)-1, and mRNA and/or protein expression/activity was measured using RT-PCR and ELISA. Neutrophil chemotactic activity in conditioned media was evaluated using migration assays. The effect of P4 was also studied.
Cyclic mechanical stretch increased IL-8, GROα, mRNA and protein, and MMP-1 production and activity level. Supernatant from stretched cells induced neutrophil chemotactic activity significantly. P4 suppressed the effect of stretch.
The current study demonstrates that cyclic mechanical stretch stimulates the production of neutrophil chemokines and MMP-1 from human decidual cells, and the production was reduced by progesterone. These findings suggest that decidual cells are responding to mechanical and endocrine signals and induce biochemical factors and thereby contribute to the regulation of human labor.