A Further Exploration of the Impact of Antinuclear Antibodies on In Vitro Fertilization–Embryo Transfer Outcome
Article first published online: 11 MAR 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 3, pages 221–229, September 2013
How to Cite
A further exploration of the impact of antinuclear antibodies on in vitro fertilization–embryo transfer outcome. Am J Reprod Immunol 2013; 70: 221–229, , , , , , , .
- Issue published online: 7 AUG 2013
- Article first published online: 11 MAR 2013
- Manuscript Accepted: 7 FEB 2013
- Manuscript Received: 5 DEC 2012
- Science& Technology Project of Guangdong Province. Grant Number: 2009B030801155
- Scientific Research Program of Population and Family-plan Committee of Guangdong Province. Grant Number: 2010243
- Antinuclear antibodies;
- in vitro fertilization–embryo transfer;
To determine the presence of antinuclear antibodies (ANAs) in follicular fluid (FF) and embryos as to their potential impact on IVF outcome.
A total of 100 women presenting to IVF/ICSI program were recruited in this study, wherein 50 women sero-positive for ANA served as study group (ANA+ group) and 50 women seronegative for ANA served as control group (ANA- group), and patients were age-matched in the two groups. ANA level in serum and FF were examined by ELISA, and immunofluorescence assay was employed to detect IgG within embryos.
In ANA+ group, 36 women showed positive ANA both in serum and FF on the day of ovum pick-up (OPU), wherein 34 exhibited fluorescence of IgG in embryos. All the 50 women in control group showed negative results in serum and FF ANA on OPU day and in embryo immunofluorescence. Serum ANA level positively correlated with FF ANA level. IVF outcomes were significantly poorer in ANA+ group, mainly reflecting the less number of high-quality embryos, reduced rates of pregnancy, clinical pregnancy, and implantation.
These data demonstrated the existence of ANA in FF and embryos, which may exert detrimental impact on IVF outcome.