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Decreased Levels of Folate Receptor-β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre-Eclampsia

Authors


  • This work was supported in part by a McKern Scholar Award for Perinatal Research (SG) and P01 Grant HD054713-01 (SG) from the NIH.

Correspondence

Seth Guller, Department of OB/GYN, Yale University School of Medicine, 333 Cedar Street-339 FMB, PO Box 208063, New Haven, CT 06520-8063, USA.

E-mail: seth.guller@yale.edu

Abstract

Problem

Pre-eclampsia (PE), a pregnancy complication of unknown etiology, is a major cause of maternal and fetal mortality and morbidity. Previous studies have described placental genes that are up-regulated in expression in PE, but few studies have addressed placental gene suppression in this syndrome.

Method of study

Gene profiling and quantitative reverse transcription PCR (qRTPCR) analyses were used to identify genes down-regulated in placentas from women with severe preterm PE compared with gestational age-matched normotensive controls with spontaneous preterm birth (sPTB). Western blotting and immunohistochemistry were used to evaluate levels and patterns of cell type–specific protein expression in PE and sPTB group placentas.

Results

Levels of macrophage marker [folate receptor (FR)-β, CD163, and CD68] mRNA and FR-β protein were significantly down-regulated in PE group placentas compared with the sPTB group. Numbers of Hofbauer cells (HBCs, fetal macrophages) and FR-β protein in these cells were reduced in PE group placentas.

Conclusion

Severe PE is associated with decreased placental expression of FR-β and a reduction in the number of HBCs. Reduced placental macrophage function is likely to play a key role in the pathophysiology of PE.

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