Effect of Culture Conditions on the Phenotype of THP-1 Monocyte Cell Line
Article first published online: 29 APR 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 1, pages 80–86, July 2013
How to Cite
Effect of culture conditions on the phenotype of THP-1 monocyte cell line. Am J Reprod Immunol 2013 70: 80–86., , , .
- Issue published online: 11 JUN 2013
- Article first published online: 29 APR 2013
- Manuscript Accepted: 1 APR 2013
- Manuscript Received: 12 MAR 2013
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- National Institutes of Health
- Department of Health and Human Services. Grant Number: P01HD054713
- PMA ;
Macrophage function has many implications in a variety of diseases. Understanding their biology becomes imperative when trying to elucidate immune cell interactions with their environment, and in vitro cell lines allow researchers to manipulate these interactions. A common cell line used is THP-1, a promyeloid cell line suggestive to outside factors, and therefore sensitive to culture conditions. In this study, we describe how culture conditions can alter THP-1 morphology and in turn affect their response to differentiation stimuli.
Method of Study
THP-1 cells were cultured in two conditions and treated with phorbol 12-myristate 13-acetate (PMA) or MCSF. CD14 surface expression was determined by flow cytometry and cytokine/chemokine production determined by multiplex analysis.
Culture conditions of THP-1 affect their response to PMA. Highly confluent THP-1 cells differentiate into a heterogeneous population responsive to PMA as seen by an increase in CD14 expression. However, these cells, cultured in low confluence, remain as a homogenous population and do not gain CD14. Additionally, there are major differences in the constitutive cytokine profile.
We demonstrate that the culture conditions of THP-1 cells can alter their response PMA. This suggests that culture techniques may account for the discrepancy in the literature of both basal THP-1 phenotype and their response to PMA.