Heat Shock Induces Interferon-TAU Gene Expression by In Vitro-Produced Bovine Blastocysts
Article first published online: 3 MAY 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 3, pages 177–181, September 2013
How to Cite
Heat shock induces interferon-tau gene expression by in vitro-produced bovine blastocysts. Am J Reprod Immunol 2013; 70: 177–181, , , , .
- Issue published online: 7 AUG 2013
- Article first published online: 3 MAY 2013
- Manuscript Accepted: 2 APR 2013
- Manuscript Received: 18 SEP 2012
- heat shock protein 70;
- in vitro production
The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro.
Method of study
In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified.
Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not.
The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.