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Keywords:

  • IL-6;
  • Implantation failure;
  • NK cells;
  • unexplained infertility;
  • VEGF

Problem

Peripheral counts of CD16NK cells have been well characterized in reproductive failure. However, not enough case-control clinical studies have been conducted to establish normal or abnormal CD16+/− values in the endometrium.

Method of study

Peripheral and endometrial NK cell counts by FACS, IL-6, and VEGF cytokines levels by ELISA were characterized in fertile women and unexplained infertility patients with implantation failures (UI-IF) during implantation window. ROC and correlation analysis were performed.

Results

Receiver Operating Characteristic(ROC) analysis revealed endometrial CD16NK cells, IL-6, and VEGF as good diagnostic parameters for unexplained infertility. Almost half of UI-FI patients showed increased total and CD16NK cell counts correlating with decreased levels of endometrial IL-6 and VEGF. No correlation was found with peripheral blood values.

Conclusion

Increased CD16NK cells were associated with IL-6 and VEGF deficiency in a high proportion of UI-IF patients. Testing for these immunomarkers could be a potential tool in infertility diagnosis.