Endometrial CD16+ and CD16− NK Cell Count in Fertility and Unexplained Infertility
Article first published online: 6 MAY 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 3, pages 182–189, September 2013
How to Cite
Endometrial CD16+ and CD16− NK cell count in fertility and unexplained infertility. Am J Reprod Immunol 2013; 70: 182–189, , , , , .
- Issue published online: 7 AUG 2013
- Article first published online: 6 MAY 2013
- Manuscript Accepted: 3 APR 2013
- Manuscript Received: 12 NOV 2012
- Implantation failure;
- NK cells;
- unexplained infertility;
Peripheral counts of CD16+ NK cells have been well characterized in reproductive failure. However, not enough case-control clinical studies have been conducted to establish normal or abnormal CD16+/− values in the endometrium.
Method of study
Peripheral and endometrial NK cell counts by FACS, IL-6, and VEGF cytokines levels by ELISA were characterized in fertile women and unexplained infertility patients with implantation failures (UI-IF) during implantation window. ROC and correlation analysis were performed.
Receiver Operating Characteristic(ROC) analysis revealed endometrial CD16+ NK cells, IL-6, and VEGF as good diagnostic parameters for unexplained infertility. Almost half of UI-FI patients showed increased total and CD16+ NK cell counts correlating with decreased levels of endometrial IL-6 and VEGF. No correlation was found with peripheral blood values.
Increased CD16+ NK cells were associated with IL-6 and VEGF deficiency in a high proportion of UI-IF patients. Testing for these immunomarkers could be a potential tool in infertility diagnosis.