TLR7 Expression is Decreased During Tumour Progression in Transgenic Adenocarcinoma of Mouse Prostate Mice and Its Activation Inhibits Growth of Prostate Cancer Cells
Article first published online: 24 JUN 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 4, pages 317–326, October 2013
How to Cite
TLR7 expression is decreased during tumour progression in transgenic adenocarcinoma of mouse prostate mice and its activation inhibits growth of prostate cancer cells. Am J Reprod Immunol 2013; 70: 317–326, , , , , , , , , .
- Issue published online: 13 SEP 2013
- Article first published online: 24 JUN 2013
- Manuscript Accepted: 4 JUN 2013
- Manuscript Received: 8 FEB 2013
- Basic Research in Science and Engineering. Grant Number: 2010-0002626
- World Class Institute (WCI). Grant Number: 2009-002
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science, and Technology of Korea (MEST)
- Colony formation;
- growth inhibition;
- prostate cancer;
- transgenic adenocarcinoma of mouse prostate mice
Although various Toll-like receptors (TLRs) have been associated with immune response and tumorigenesis in the prostate cells, little is known about the role of TLR7. Accordingly, we examined the expression of TLR7 during tumour progression of TRMAP (transgenic mouse model for prostate cancer) mice and its role on cell growth.
Method of study
Toll-like receptor7 expression was examined by RT-polymerase chain reaction (PCR), Western blot, and immunohistochemistry. Cell growth was examined by MTT assay. Colony formation was investigated by crystal violet staining.
Strong expression of TLR7 was detected in the normal prostate epithelia of Wild-type (WT) mice, but not in TLR7-deficient mice. In contrast, TLR7 expression was weak in transgenic adenocarcinoma of mouse prostate (TRAMP)-C2 cells, as compared with murine bone marrow-derived macrophages (BMDMs). Moreover, TLR7 mRNA was markedly expressed in RWPE-1 cells (non-cancerous prostate epithelial cells), but not in PC3 and DU145 (prostate cancer cells). Immunohistochemically, TLR7 expression gradually decreased in TRAMP mice depending on the pathologic grade of the prostate cells. TLR7 agonists increased both the gene and protein expression of TLR7 and promoted production of proinflammatory cytokines/chemokines and IFN-β gene expression in prostate cancer cell lines. Moreover, loxoribine inhibited the growth and colony formation of TRAMP-C2 cells dependent of TLR7.
These findings suggest that TLR7 may participate in tumour suppression in the prostate cells.