The Gene Expression Analysis of Paracrine/Autocrine Factors in Patients with Spermatogenetic Failure Compared with Normal Spermatogenesis
Article first published online: 19 JUL 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 6, pages 522–528, December 2013
How to Cite
The gene expression analysis of paracrine/autocrine factors in patients with spermatogenetic failure compared with normal spermatogenesis. Am J Reprod Immunol 2013; 70: 522–528, , , , , .
- Issue published online: 14 NOV 2013
- Article first published online: 19 JUL 2013
- Manuscript Accepted: 13 JUN 2013
- Manuscript Received: 10 APR 2013
- The National Centre for Research and Development. Grant Number: N R13 0066 06
The aim of this study was to examine the expression levels of IL-1 family members, IL-6, IL-10, TNF family, SCF, and c-kit in infertile patients with idiopathic non-obstructive azoospermia (NOA) compared with men with normal spermatogenesis.
Method of study
We analyzed 20 testicular biopsy samples with Affymetrix Human Gene 1.0 ST microarrays (Affymetrix, Santa Clara, CA, USA). Sixteen of them were obtained from patients with various types of NOA and four with normal spermatogenesis.
The comparative analysis of normal and pathological group demonstrated a different expression level of IL1-RA gene. It was also observed that the gene expression levels for IL1-R1, CASP1, and stem cell factor (SCF) were upregulated in the Sertoli-cell-only syndrome group in comparison with the control one (P < 0.05).
The microarray analysis showed the expression level of all investigated paracrine/autocrine factors at one go, and therefore, the possible interaction between these genes could be examined.