These authors contributed equally to this work.
Thymic Stromal Lymphopoietin Suppresses the Apoptosis of Decidual Gamma-delta T Cells via Regulation of the Signal Transduction and Activation of Transcription 3/Caspase-3 Signaling Pathway
Article first published online: 13 SEP 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 70, Issue 6, pages 464–471, December 2013
How to Cite
Thymic stromal lymphopoietin suppresses the apoptosis of decidual gamma-delta T cells via regulation of the STAT3/Caspase-3 signaling pathway. Am J Reprod Immunol 2013; 70: 464–471, , , , , , .
- Issue published online: 14 NOV 2013
- Article first published online: 13 SEP 2013
- Manuscript Accepted: 16 AUG 2013
- Manuscript Received: 6 MAY 2013
- National Natural Science Foundation of China. Grant Number: 31170870
- signal transduction and activation of transcription 3;
- thymic stromal lymphopoietin;
- γδ T cells
To investigate whether thymic stromal lymphopoietin (TSLP) regulates the apoptosis of decidual γδ T cells and to elucidate the mechanism.
Method of study
Primary human decidual γδ T cells were treated with TSLP only or TSLP combined with different signaling inhibitors (STAT3, STAT5, AKT, and ERK). The levels of signal transduction and activation of transcription 3 (STAT3) tyrosine phosphorylation and caspase3 expression were determined using Western blot analysis, and the apoptosis of decidual γδ T cells was analyzed by flow cytometry.
The proportions of γδ T cells in the peripheral circulation and in decidual CD3+ cell population in women with normal pregnancy were higher than the proportions of γδ T cells in either non-pregnant control or miscarriage. Decidual γδ T cells co-expressed the TSLP receptors (TSLPR) and IL-7Rα, and the expression of TSLPR in decidual γδ T cells was higher than that in decidual CD8+ and CD4+ T cells. Treatment with TSLP significantly suppressed the apoptosis of decidual γδ T cells and enhanced STAT3 phosphorylation. Moreover, STAT3, and not other inhibitors, completely abrogated the anti-apoptotic effect and expression of caspase3 in decidual γδ T cells induced by recombinant human TSLP.
These results suggest that TSLP may down-regulate caspase3 expression through activation of the STAT3 pathway, thereby suppressing the apoptosis of decidual γδ T cells.