Thymic Stromal Lymphopoietin Suppresses the Apoptosis of Decidual Gamma-delta T Cells via Regulation of the Signal Transduction and Activation of Transcription 3/Caspase-3 Signaling Pathway

Authors

  • Jie Duan,

    1. Laboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Xiao-Ping Jiang,

    1. Department of Gynecology and Obstetrics, Shanghai Corps Hospital of Chinese People's Armed Police Forces, Shanghai, China
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Ming-Qing Li,

    1. Laboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China
    Search for more papers by this author
  • Deng-Xuan Fan,

    1. Laboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China
    Search for more papers by this author
  • Ying Wang,

    1. Laboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China
    Search for more papers by this author
  • Da-Jin Li,

    Corresponding author
    1. Laboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China
    • Correspondence

      Da-Jin Li or Li-Ping Jin, Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China.

      E-mails: djli@shmu.edu.cn or zjplp2004@yahoo.com.cn

    Search for more papers by this author
  • Li-Ping Jin

    Corresponding author
    1. Laboratory for Reproductive Immunology, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China
    • Correspondence

      Da-Jin Li or Li-Ping Jin, Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China.

      E-mails: djli@shmu.edu.cn or zjplp2004@yahoo.com.cn

    Search for more papers by this author

Abstract

Problem

To investigate whether thymic stromal lymphopoietin (TSLP) regulates the apoptosis of decidual γδ T cells and to elucidate the mechanism.

Method of study

Primary human decidual γδ T cells were treated with TSLP only or TSLP combined with different signaling inhibitors (STAT3, STAT5, AKT, and ERK). The levels of signal transduction and activation of transcription 3 (STAT3) tyrosine phosphorylation and caspase3 expression were determined using Western blot analysis, and the apoptosis of decidual γδ T cells was analyzed by flow cytometry.

Results

The proportions of γδ T cells in the peripheral circulation and in decidual CD3+ cell population in women with normal pregnancy were higher than the proportions of γδ T cells in either non-pregnant control or miscarriage. Decidual γδ T cells co-expressed the TSLP receptors (TSLPR) and IL-7Rα, and the expression of TSLPR in decidual γδ T cells was higher than that in decidual CD8+ and CD4+ T cells. Treatment with TSLP significantly suppressed the apoptosis of decidual γδ T cells and enhanced STAT3 phosphorylation. Moreover, STAT3, and not other inhibitors, completely abrogated the anti-apoptotic effect and expression of caspase3 in decidual γδ T cells induced by recombinant human TSLP.

Conclusion

These results suggest that TSLP may down-regulate caspase3 expression through activation of the STAT3 pathway, thereby suppressing the apoptosis of decidual γδ T cells.

Ancillary