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Progesterone Elicits an Inhibitory Effect upon LPS-Induced Innate Immune Response in Pre-Labor Human Amniotic Epithelium

Authors

  • Pilar Flores-Espinosa,

    1. Department of Cell Biology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
    2. Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
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  • Montzerrat Pineda-Torres,

    1. Department of Cell Biology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Rodrigo Vega-Sánchez,

    1. Department of Nutrition Research, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Guadalupe Estrada-Gutiérrez,

    1. Department of Immunobiochemistry, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Aurora Espejel-Nuñez,

    1. Department of Immunobiochemistry, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Arturo Flores-Pliego,

    1. Department of Immunobiochemistry, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Rolando Maida-Claros,

    1. Department of Neonatology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Yuriria Paredes-Vivas,

    1. Department of Cell Biology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Iyari Morales-Méndez,

    1. Department of Infectology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Irma Sosa-González,

    1. Department of Infectology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
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  • Angel Chávez-Mendoza,

    1. Department of Periodontology, Centro Interdisciplinario de Ciencias de la Salud, Instituto Politecnico Nacional, Mexico City, Mexico
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  • Verónica Zaga-Clavellina

    Corresponding author
    1. Department of Cell Biology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico
    • Correspondence

      Verónica Zaga-Clavellina, Instiuto Nacional de Perinatología, Isidro Espinosa de los Reyes, Montes Urales 800, Lomas Virreyes, D.F. 11000, México.

      E-mail: sciencefeedback@gmail.com

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Abstract

Problem

Infection of human fetal membranes elicits secretion of pro-inflammatory modulators through its innate immune capacities. We investigated the effect of lipopolysacharide (LPS) and progesterone (P4) upon expression of TLR-4/MyD88, TNFα, IL-6, IL-8, IL-10, and HBD2 on the human amniotic epithelium.

Method of study

Explants of the human amniotic epithelium were pre-treated with 0.01, 0.1, and 1.0 μm of P4; then cotreated with 1000 ng/mL LPS. TLR-4 was immuno-detected, and concentrations of MyD88, TNFα, IL-6, IL-8, IL-10, and HBD2 were quantified by ELISA.

Results

P4 significantly reduced the expression of LPS-induced TLR-4/MyD88. LPS increased the concentrations of TNFα, IL-6, IL-8, IL-10, and HBD2 by factors of 30-, eight, three, three, and fivefold, respectively. P4 at 1.0 μm was the most effective dose to blunt the secretion of TNFα, IL-6, and HBD-2. RU-486 blocks the effect of P4.

Conclusion

P4 inhibited LPS-induced TLR-4/MyD88 and pro-inflammatory factors in the human amniotic epithelium. These results could explain partially how P4 can protect the amniotic region of fetal membranes and generate a compensatory mechanism that limits the secretion of pro-inflammatory modulators, which could jeopardize the immune privilege during pregnancy.

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