Choriodecidual Cells From Term Human Pregnancies Show Distinctive Functional Properties Related to the Induction of Labor
Article first published online: 29 NOV 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 71, Issue 1, pages 86–93, January 2014
How to Cite
Choriodecidual cells from term human pregnancies show distinctive functional properties related to the induction of labor. Am J Reprod Immunol 2014; 71: 86–93, , , , , , .
- Issue published online: 11 DEC 2013
- Article first published online: 29 NOV 2013
- Manuscript Accepted: 16 OCT 2013
- Manuscript Received: 25 JUL 2013
- U.S. National Institute for Environmental Health Sciences. Grant Number: R01 ES016932
- National Institutes of Health
- National Council of Science and Technology (CONACyT)
- U.N.A.M.. Grant Number: PAPIIT IA200612-2
- Wayne State University Research Initiative in Maternal, Perinatal, and Child health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health
- fetal membranes;
Human parturition is associated with an intrauterine pro-inflammatory environment in the choriodecidua. Evidence that some mediators of this signaling cascade also elicit responses leading to labor prompted us to characterize the cellular sources of these mediators in the human choriodecidua.
Method of study
Leukocyte-enriched preparations from human choriodecidua (ChL) and intervillous placental blood leukocytes (PL) were maintained in culture. Secretions of inflammatory cytokines, chemokines, and MMP-9 were documented. Leukocyte phenotype of ChL and PL was determined by flow cytometry using specific fluorochrome-conjugated antibodies.
Results and Conclusions
ChL showed a distinct pro-inflammatory secretion pattern of cytokines and chemokines when compared with PL, including higher amounts of TNF-α and IL-6, and decreased secretions of IL-4 and IL-1ra. ChL also secreted more MIP-1α and MCP-1 and MMP-9 than PL. No significant differences were found in leukocytes subsets between compartments. Based on our findings, we propose that ChL isolated from fetal membranes at term are functionally different from PL and may collaborate to modulate the microenvironment linked to induction and progression of human labor.