Get access

Choriodecidual Cells From Term Human Pregnancies Show Distinctive Functional Properties Related to the Induction of Labor

Authors

  • Marisol Castillo-Castrejon,

    1. Unidad de Vinculación de la Facultad de Medicina, U.N.A.M., Instituto Nacional de Medicina Genómica, Mexico City, Mexico
    2. Programa de Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
    Search for more papers by this author
  • Noemí Meraz-Cruz,

    1. Unidad de Vinculación de la Facultad de Medicina, U.N.A.M., Instituto Nacional de Medicina Genómica, Mexico City, Mexico
    Search for more papers by this author
  • Nardhy Gomez-Lopez,

    1. Departments of Obstetrics and Gynecology & Immunology and Microbiology, School of Medicine, Wayne State University, Perinatology Research Branch/NICHD/NIH, Detroit, MI, USA
    Search for more papers by this author
  • Arturo Flores-Pliego,

    1. Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    Search for more papers by this author
  • Jorge Beltrán-Montoya,

    1. Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico
    Search for more papers by this author
  • Martín Viveros-Alcaráz,

    1. Hospital Materno Infantil Inguarán, Secretaría de Salud del D.F., Mexico City, Mexico
    Search for more papers by this author
  • Felipe Vadillo-Ortega

    Corresponding author
    1. Unidad de Vinculación de la Facultad de Medicina, U.N.A.M., Instituto Nacional de Medicina Genómica, Mexico City, Mexico
    • Correspondence

      Felipe Vadillo-Ortega, Unidad de Vinculación de la Facultad de Medicina, U.N.A.M., Instituto Nacional de Medicina Genómica, Periférico Sur 4809, Col. Arenal Tepepan, Del. Tlalpan, C.P.14610, Mexico city, Mexico.

      E-mail: felipe.vadillo@gmail.com

    Search for more papers by this author

Abstract

Problem

Human parturition is associated with an intrauterine pro-inflammatory environment in the choriodecidua. Evidence that some mediators of this signaling cascade also elicit responses leading to labor prompted us to characterize the cellular sources of these mediators in the human choriodecidua.

Method of study

Leukocyte-enriched preparations from human choriodecidua (ChL) and intervillous placental blood leukocytes (PL) were maintained in culture. Secretions of inflammatory cytokines, chemokines, and MMP-9 were documented. Leukocyte phenotype of ChL and PL was determined by flow cytometry using specific fluorochrome-conjugated antibodies.

Results and Conclusions

ChL showed a distinct pro-inflammatory secretion pattern of cytokines and chemokines when compared with PL, including higher amounts of TNF-α and IL-6, and decreased secretions of IL-4 and IL-1ra. ChL also secreted more MIP-1α and MCP-1 and MMP-9 than PL. No significant differences were found in leukocytes subsets between compartments. Based on our findings, we propose that ChL isolated from fetal membranes at term are functionally different from PL and may collaborate to modulate the microenvironment linked to induction and progression of human labor.

Ancillary