Phenotype and Functionality of CD4+ and CD8+ T Cells in the Upper Reproductive Tract of Healthy Premenopausal Women
Article first published online: 6 DEC 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 71, Issue 2, pages 95–108, February 2014
How to Cite
Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women. Am J Reprod Immunol 2014; 71: 95–108, , , , , , , .
- Issue published online: 10 JAN 2014
- Article first published online: 6 DEC 2013
- Manuscript Accepted: 29 OCT 2013
- Manuscript Received: 18 JUL 2013
- NIH/NIAID. Grant Number: P01 AI083050
- CTL ;
- HIV ;
- STD ;
The goal of this study was to investigate the phenotype and functional responsiveness of CD4+ and CD8+ T-cells in the upper reproductive tract of healthy premenopausal women. The lower reproductive tract is frequently studied as a site of sexually transmitted infections; however, the upper reproductive tract may also be a portal of entry and dissemination for pathogens, including HIV-1.
Method of Study
Endometrial biopsy, endocervical curettage, cytobrush, and blood were collected during mid-luteal phase from 23 healthy women. T-cells were isolated and analyzed by flow cytometry.
As compared with their counterparts in blood, endometrial and endocervical T-cells had enhanced CCR5 expression, and were enriched for activated, effector memory cells. Endometrial T-cells were more responsive to polyclonal stimuli, producing a broad range of cytokines and chemokines.
These findings underscore the responsiveness of endometrial T-cells to stimulation, and reveal their activated phenotype. These findings also suggest susceptibility of the upper reproductive tract to HIV-1 infection.