A. Bogdan and B. Polgar contributed equally to the work.
Progesterone Induced Blocking Factor Isoforms in Normal and Failed Murine Pregnancies
Article first published online: 11 DEC 2013
© 2013 John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 71, Issue 2, pages 131–136, February 2014
How to Cite
Progesterone induced blocking factor isoforms in normal and failed murine pregnancies. Am J Reprod Immunol 2014; 71: 131–136, , .
- Issue published online: 10 JAN 2014
- Article first published online: 11 DEC 2013
- Manuscript Accepted: 3 NOV 2013
- Manuscript Received: 27 AUG 2013
- Hungarian National Research Fund. Grant Number: OTKA 77717
- University of Pecs. Grant Number: 34039/KA-PostDoc12-03
- Hungarian Ministry of Health. Grant Number: ETT 286/2009
- Research Team on Innovation. Grant Numbers: SROP-4.2.2/08/1/2008-0011, TÁMOP-4.2.1/B-10/1-2010-0002, TÁMOP-4.2.2.A-11/1/KONV-2012-0053
- mouse pregnancy;
- PIBF isoforms;
Progesterone induced blocking factor (PIBF) is required for successful pregnancy. Alternative splicing produces PIBF isoforms with different functions. The full-length (90 kDa) PIBF is involved in cell cycle regulation, whereas smaller secreted forms act as cytokines. We aim to examine the PIBF exon pattern and protein isoform profile in normal and failed murine pregnancies.
Method of study
Pregnant Balb/c mice were killed on gestation days 12–14 or 17–19. Normal and resorbed fetuses, placentae, and uterine tissue were used for RNA and protein analysis with RT-PCR and Western blot, respectively.
Late pregnancy and resorption were associated with lower expression of the N-terminal exons, together with significantly reduced production of the full-length protein.
Reduced production of the full-length PIBF protein might result in disturbed cell cycle regulation and dysregulated trophoblast invasion, while the absence of PIBF isoforms containing exon 2–4 coded sequences might lead to the loss of local immunosuppression.