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Maternal Circulating Dendritic Cell Subtypes at Delivery and During the 1-Year Postpartum Period

Authors

  • Hye Ryun Lee,

    1. Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea
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  • Byoung Jae Kim,

    1. Department of Obstetrics and Gynecology, Boramae Hospital, Seoul, Korea
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  • Sue Shin,

    Corresponding author
    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    2. Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea
    3. Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea
    • Correspondence

      Sue Shin, Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea.

      E-mail: jeannie@snu.ac.kr

      and

      Hye Won Jeon, Department of Obstetrics and Gynecology, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea.

      E-mail: ljhw@snu.ac.kr

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  • Hye Won Jeon,

    Corresponding author
    1. Department of Obstetrics and Gynecology, Boramae Hospital, Seoul, Korea
    • Correspondence

      Sue Shin, Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea.

      E-mail: jeannie@snu.ac.kr

      and

      Hye Won Jeon, Department of Obstetrics and Gynecology, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea.

      E-mail: ljhw@snu.ac.kr

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  • Eun Youn Roh,

    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    2. Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea
    3. Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea
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  • Jong Hyun Yoon,

    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    2. Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea
    3. Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea
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  • Eun Young Song

    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
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  • Lee HR and Kim BJ contributed equally to this study as co-first authors.
  • Shin S and Jeon HW contributed equally to this study as co-corresponding authors.

Abstract

Problem

Dendritic cells (DCs) play an important role in maintaining pregnancy by inducing tolerance toward the fetus. Such an immunologic change in the mother should be restored to normal after delivery, but few studies have reported postpartum maternal immune recovery, in terms of the types circulating DCs.

Method of study

The level of each DC subtype and HLA-DR-positive immunoreactivity of the blood from 29 pregnant women with uncomplicated labor was serially analyzed by flowcytometry at delivery and at 1.5, 6, and 12 months after delivery. DC subtypes were characterized as myeloid, lymphoid, and less differentiated (ldDC). Mean fluorescence intensity (MFI) was evaluated for HLA-DR expression for each DC subtype.

Results

The total number and the percentage of DCs at delivery were lower than those at 12 months postpartum. The ldDC fractions were significantly higher at delivery and at 1.5 months than at 12 months postpartum. The MFI of HLA-DR expression on ldDCs at delivery was lower than that at 12 months postpartum. The myeloid-to-lymphoid DC ratio did not differ over the 1-year postpartum period.

Conclusion

The maternal alteration in DCs rapidly normalized within 1.5 months, except for the ldDC fraction, which persisted between 1.5 and 6 months after delivery.

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