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Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR-1

Authors

  • Qiang Fu,

    1. Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai, China
    2. Department of Immunology, Binzhou Medical University, Shandong, China
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  • Yu Tao,

    1. Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai, China
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  • Hailan Piao,

    1. Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai, China
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  • Mei-Rong Du,

    Corresponding author
    1. Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai, China
    • Correspondence

      Mei-Rong Du and Da-Jin Li, Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai 200011, China.

      E-mails: dmrlq1973@sina.cn (MR Du); djli@shmu.edu.cn (DJ Li)

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  • Da-Jin Li

    Corresponding author
    1. Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai, China
    • Correspondence

      Mei-Rong Du and Da-Jin Li, Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Shanghai Medical School, Fudan University, Shanghai 200011, China.

      E-mails: dmrlq1973@sina.cn (MR Du); djli@shmu.edu.cn (DJ Li)

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Abstract

Problem

To determine the effect of collagen from maternal–fetal interface on decidual natural killer cell (dNK) function.

Method of study

Decidual and villous samples were collected from normal pregnancy and miscarriage. The phenotype and cytokine production were analyzed, respectively, by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Co-culture was established to investigate the effect of trophoblasts and decidual stromal cells (DSCs) on dNKs.

Results

Maternal–fetal interface of normal pregnancy showed higher collagen and LAIR-1 expression than that of miscarriage. Co-culture of dNKs with HTR-8/DSCs up-regulated LAIR-1 on dNKs that could be attenuated by pre-treatment with LAIR-2, a competitive inhibitor of LAIR-1. Collagen down-regulated expression of cell surface receptor activity and intracellular perforin, while it up-regulated expression of suppressive receptor on dNKs. Co-culture of dNKs with HTR-8/DSCs decreased perforin expression and Th1-type cytokines production by dNKs, which could be abrogated by LAIR-2. In addition, silence of collagen in HTR-8/DSCs by shRNA significantly attenuated regulation on dNKs.

Conclusion

Trophoblasts and DSCs regulate decidual NK cell functions via secreting collagen, which is involved in the maintenance of human pregnancy.

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