Expression of 8-oxoguanine Glycosylase in Human Fetal Membranes
Article first published online: 3 MAR 2014
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 72, Issue 1, pages 75–84, July 2014
How to Cite
Expression of 8-oxoguanine glycosylase in human fetal membranes. Am J Reprod Immunol 2014; 72: 75–84, , , , .
- Issue published online: 11 JUN 2014
- Article first published online: 3 MAR 2014
- Manuscript Accepted: 27 JAN 2014
- Manuscript Received: 15 NOV 2013
- Department of Obstetrics and Gynecology
- The University of Texas Medical Branch
- Base excision repair;
- DNA damage;
- DNA repair;
- oxidative stress;
- preterm premature rupture of the membranes;
The most common DNA lesion generated by oxidative stress (OS) is 7, 8-dihydro-8-oxoguanine (8-oxoG) whose excision repair is performed by 8-oxoguanine glycosylase (OGG1). We investigated OGG1 expression changes in fetal membranes from spontaneous preterm birth (PTB) and preterm premature rupture of the membranes (pPROM) and its changes in vitro in normal fetal membranes exposed to OS inducer water-soluble cigarette smoke extract (CSE).
Method of study
DNA damage was determined in amnion cells treated with CSE by comet and FLARE assays. OGG1 mRNA expression and localization in fetal membranes from clinical specimens and in normal term membranes exposed to CSE were examined by QRT-PCR and by immunohistochemistry.
DNA strand and base damage was seen in amnion cells exposed to CSE. OGG1 expression was 2.5-fold higher in PTB samples compared with pPROM (P = 0.045). No significant difference was seen between term and pPROM or PTB and term. CSE treatment showed a nonsignificant decrease in OGG1. OGG1 was localized to both amnion and chorion with less intense staining in pPROM and CSE-treated membranes.
Increased OS-induced DNA damage predominated by 8-oxoG is likely to persist in fetal cells due to reduced availability of base excision repair enzyme OGG1. This can likely lead to fetal cell senescence associated with some adverse pregnancy outcome.