Transcobalamin II (TCN2 67A>G and TCN2 776C>G) and Transcobalamin II Receptor (TCblR 1104C>T) Polymorphisms in Korean Patients with Idiopathic Recurrent Spontaneous Abortion

Authors

  • Hyun Seok Kim,

    1. Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
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  • Bo Eun Lee,

    1. Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
    2. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea
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  • Young Joo Jeon,

    1. Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
    2. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea
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  • HyungChul Rah,

    1. Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
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  • Woo Sik Lee,

    1. Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul, South Korea
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  • Ji Eun Shin,

    1. Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
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  • Dong Hee Choi,

    Corresponding author
    1. Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
    • Correspondence

      Nam Keun Kim, PhD, Institute for Clinical Research, CHA Bundang Medical Center, CHA University, 351, Yatap-dong, Bundang-gu, Seongnam 463-712, South Korea.

      E-mail: nkkim@cha.ac.kr

      and

      Dong Hee Choi, MD, PhD

      Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seoul 135-081, South Korea.

      E-mail: dhchoimc@cha.ac.kr

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  • Nam Keun Kim

    Corresponding author
    1. Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea
    2. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea
    • Correspondence

      Nam Keun Kim, PhD, Institute for Clinical Research, CHA Bundang Medical Center, CHA University, 351, Yatap-dong, Bundang-gu, Seongnam 463-712, South Korea.

      E-mail: nkkim@cha.ac.kr

      and

      Dong Hee Choi, MD, PhD

      Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seoul 135-081, South Korea.

      E-mail: dhchoimc@cha.ac.kr

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Abstract

Problem

The transcobalamin II (TCN2) 776C>G polymorphism has been reported to be a genetic risk factor for idiopathic recurrent spontaneous abortion (RSA). However, the sample size in previous studies was small, and other TCN2 polymorphisms have not been studied. Moreover, the TCN2 67A>G and 776C>G polymorphisms, and the transcobalamin II receptor (TCblR/CD320) 1104C>T polymorphism, have demonstrated associations with immune responses.

Method of study

Three hundred and seventy-eight RSA patients who had at least two consecutive spontaneous abortions were enrolled. Two hundred and seven control subjects were collected from a convenience sample. Polymerase chain reaction and restriction fragment length polymorphism analysis were performed to identify the TCN2 67A>G and 776C>G polymorphisms, and the TCblR 1104C>T polymorphism.

Results

RSA patients showed significantly different frequencies of the TCN2 67AG+GG genotypes compared with control subjects.

Conclusion

The TCN2 67G allele is a possible risk factor for idiopathic RSA.

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