Conjugated Linoleic Acids Attenuate LPS-Induced Pro-Inflammatory Gene Expression by Inhibiting the NF-κB Translocation Through PPARγ in Buffalo Granulosa Cells
Article first published online: 5 MAY 2014
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 72, Issue 3, pages 296–304, September 2014
How to Cite
Conjugated linoleic acids attenuate LPS-induced pro-inflammatory gene expression by inhibiting the NF-κB translocation through PPARγ in buffalo granulosa cells. Am J Reprod Immunol 2014; 72: 296–304, , .
- Issue published online: 7 AUG 2014
- Article first published online: 5 MAY 2014
- Manuscript Accepted: 31 MAR 2014
- Manuscript Received: 16 NOV 2013
In granulosa cells, TLR4-mediated LPS-induced immune response interferes with ovarian granulosa cell function.
Method of study
LPS-induced pro-inflammatory gene expression was monitored by real-time PCR and NF-κB was determined by the immuno-blotting and immuno-staining in granulosa cells in vitro.
The LPS (1 μg/mL) increased pro-inflammatory gene expression which was reverted back by treatment with TLR4 signaling inhibitor. Cotreatment of CLA (10 μm) with LPS did not show any effect on LPS-induced pro-inflammatory gene expression but granulosa cells pre-treated with CLA for 24 hr, attenuated LPS-induced pro-inflammatory gene expression and nuclear NF-κB. GW9662, a PPARγ-inhibitor, further increased the expression of pro-inflammatory genes.
The present findings reiterated that pre-treatment with CLA can prevent LPS-induced granulosa cells dysfunction.