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Conjugated Linoleic Acids Attenuate LPS-Induced Pro-Inflammatory Gene Expression by Inhibiting the NF-κB Translocation Through PPARγ in Buffalo Granulosa Cells

Authors

  • Vengala Rao Yenuganti,

    1. Molecular Endocrinology Laboratory, Animal Biochemistry Division, National Dairy Research Institute, Karnal, India
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  • Ravinder Ravinder,

    1. Molecular Endocrinology Laboratory, Animal Biochemistry Division, National Dairy Research Institute, Karnal, India
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  • Dheer Singh

    Corresponding author
    1. Molecular Endocrinology Laboratory, Animal Biochemistry Division, National Dairy Research Institute, Karnal, India
    • Correspondence

      Dheer Singh, Molecular Endocrinology Laboratory, Animal Biochemistry Division, National Dairy Research Institute (ICAR), Karnal-132001, Haryana, India.

      E-mail: drdheer.singh@gmail.com

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Abstract

Problem

In granulosa cells, TLR4-mediated LPS-induced immune response interferes with ovarian granulosa cell function.

Method of study

LPS-induced pro-inflammatory gene expression was monitored by real-time PCR and NF-κB was determined by the immuno-blotting and immuno-staining in granulosa cells in vitro.

Results

The LPS (1 μg/mL) increased pro-inflammatory gene expression which was reverted back by treatment with TLR4 signaling inhibitor. Cotreatment of CLA (10 μm) with LPS did not show any effect on LPS-induced pro-inflammatory gene expression but granulosa cells pre-treated with CLA for 24 hr, attenuated LPS-induced pro-inflammatory gene expression and nuclear NF-κB. GW9662, a PPARγ-inhibitor, further increased the expression of pro-inflammatory genes.

Conclusion

The present findings reiterated that pre-treatment with CLA can prevent LPS-induced granulosa cells dysfunction.

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