Extracellular Adenosine Triphosphate Affects Systemic and Kidney Immune Cell Populations in Pregnant Rats
Version of Record online: 8 MAY 2014
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 72, Issue 3, pages 305–316, September 2014
How to Cite
Extracellular adenosine triphosphate affects systemic and kidney immune cell populations in pregnant rats. Am J Reprod Immunol 2014; 72: 305–316, , , , , , , .
- Issue online: 7 AUG 2014
- Version of Record online: 8 MAY 2014
- Manuscript Accepted: 9 APR 2014
- Manuscript Received: 20 DEC 2013
- Dutch Kidney Foundation. Grant Number: C08.2266
- Dutch Technology Foundation STW. Grant Number: 10704
- Extracellular ATP;
- rat model
Changes in the systemic immune response are found in preeclampsia. This may be related to high extracellular adenosine triphosphate (ATP) levels. The question arose whether ATP could affect immune responses in pregnancy. Previously, we investigated whether ATP affected monocyte activation and subpopulations. Here, we investigated ATP-induced changes in other immune cell populations in pregnant rats, systemically and in the kidney, an affected organ in preeclampsia.
Method of study
Using flow cytometry or immunohistochemistry, blood and kidney leukocytes were studied in pregnant and non-pregnant rats at different intervals after ATP or saline infusion.
Adenosine triphosphate (ATP) infusion induced increased peripheral blood non-classical monocytes and decreased T lymphocyte subsets in pregnant rats only, higher glomerular macrophage and T lymphocyte numbers in non-pregnant animals 1 day after infusion, and higher glomerular macrophage numbers in pregnant rats 6 days after infusion.
Adenosine triphosphate (ATP) infusion in pregnant rats induced a pregnancy-specific inflammatory response. Increased ATP levels could potentially contribute to development of the inflammatory response of preeclampsia.