Testosterone Therapy Differently Regulates the Anti- and Pro-Inflammatory Cytokines in the Plasma and Prostate of Rats Submitted to Chronic Ethanol Consumption (UChB)
Article first published online: 15 MAY 2014
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 72, Issue 3, pages 317–325, September 2014
How to Cite
Testosterone therapy differently regulates the anti- and pro-inflammatory cytokines in the plasma and prostate of rats submitted to chronic ethanol consumption (UChB). Am J Reprod Immunol 2014; 72: 317–325, , , , , .
- Issue published online: 7 AUG 2014
- Article first published online: 15 MAY 2014
- Manuscript Accepted: 9 APR 2014
- Manuscript Received: 14 MAR 2014
- Research Foundation of Sao Paulo State. Grant Numbers: 2009/54606-1, 2011/05936-9, 2012/00917-9
Ethanol consumption damages the prostate, and testosterone is known by anti-inflammatory role.
Methods of Study
The cytokines were investigated in the plasma and ventral prostate of UChB rats submitted or not to testosterone therapy by ELISA and Western blot, respectively. Additionally, inflammatory foci and mast cells were identified in the ventral prostate slides stained by hematoxylin and eosin and toluidine blue, respectively.
Inflammatory foci were found in the ethanol-treated animals and absent after testosterone therapy. Plasma levels of IL-6 and IL-10 were not changed while TNFα and TFG-β1 were increased in the animals submitted testosterone therapy. Regarding to ventral prostate, IL-6 did not alter, while IL-10, TNFα, and TFG-β1 were increased after testosterone therapy. Ethanol increases NFR2 in addition to high number of intact and degranulated mast cell which were reduced after testosterone therapy.
So, ethanol and testosterone differentially modulates the cytokines in the plasma and prostate.