Testosterone Therapy Differently Regulates the Anti- and Pro-Inflammatory Cytokines in the Plasma and Prostate of Rats Submitted to Chronic Ethanol Consumption (UChB)

Authors

  • Leonardo Oiveira Mendes,

    1. Structural and Cell Biology Program, State University of Campinas – UNICAMP, Campinas/SP, Brazil
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  • Wellerson Rodrigo Scarano,

    1. Department of Morphology, Institute of Biosciences, UNESP – Univ Estadual Paulista, Botucatu/SP, Brazil
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  • Sabrina Santos Rochel-Maia,

    1. Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto/SP, Brazil
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  • Beatriz Aparecida Fioruci-Fontaneli,

    1. Structural and Cell Biology Program, State University of Campinas – UNICAMP, Campinas/SP, Brazil
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  • Luiz Gustavo Almeida Chuffa,

    1. Department of Anatomy, Institute of Biociences, UNESP – Univ Estadual Paulista, Botucatu/SP, Brazil
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  • Francisco Eduardo Martinez

    Corresponding author
    1. Department of Anatomy, Institute of Biociences, UNESP – Univ Estadual Paulista, Botucatu/SP, Brazil
    • Correspondence

      Francisco Eduardo Martinez, Department of Anatomy, Institute of Bioscience, UNESP - Univ Estadual Paulista, PO Box 510, Rubião Junior, s/n, 18618-910, Botucatu/SP, Brazil.

      E-mail: martinez@ibb.unesp.br

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Abstract

Problem

Ethanol consumption damages the prostate, and testosterone is known by anti-inflammatory role.

Methods of Study

The cytokines were investigated in the plasma and ventral prostate of UChB rats submitted or not to testosterone therapy by ELISA and Western blot, respectively. Additionally, inflammatory foci and mast cells were identified in the ventral prostate slides stained by hematoxylin and eosin and toluidine blue, respectively.

Results

Inflammatory foci were found in the ethanol-treated animals and absent after testosterone therapy. Plasma levels of IL-6 and IL-10 were not changed while TNFα and TFG-β1 were increased in the animals submitted testosterone therapy. Regarding to ventral prostate, IL-6 did not alter, while IL-10, TNFα, and TFG-β1 were increased after testosterone therapy. Ethanol increases NFR2 in addition to high number of intact and degranulated mast cell which were reduced after testosterone therapy.

Conclusions

So, ethanol and testosterone differentially modulates the cytokines in the plasma and prostate.

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