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The Anti-Inflammatory Impact of Omega-3 Polyunsaturated Fatty Acids During the Establishment of Endometriosis-Like Lesions

Authors

  • Jill A. Attaman,

    1. Department of Obstetrics and Gynecology, Harvard Vanguard Medical Associates, Boston, MA, USA
    2. Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
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    • JA Attaman and AK Stanic contributed equally to this work.
  • Aleksandar K. Stanic,

    1. Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
    2. Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
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    • JA Attaman and AK Stanic contributed equally to this work.
  • Minji Kim,

    1. Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
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  • Maureen P. Lynch,

    1. Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
    2. Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
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  • Bo R. Rueda,

    1. Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
    2. Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
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  • Aaron K. Styer

    Corresponding author
    1. Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
    2. Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
    • Correspondence

      Aaron K. Styer, Vincent Center for Reproductive Biology, Thier 9, Massachusetts General Hospital, Boston, MA 02114, USA.

      E-mail: astyer@partners.org

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Abstract

Problem

The anti-inflammatory impact of three polyunsaturated fatty acids (3-PUFA) in endometriosis is incompletely understood. The effect of 3-PUFA on endometriosis-like lesions is evaluated as a potential anti-inflammatory treatment target.

Method of Study

Wild Type (WT) and transgenic Fat-1 mice (high levels of endogenous 3-PUFA) were utilized in a uterine tissue transplant endometriosis model. Experimental donor × host pairs included: WT × WT (WW), WT × Fat-1 (WF), and Fat-1 × Fat-1 (FF). Cytokine content (IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-17A, IFN-γ, TNF-γ, MCP-1 and RANTES) and immunocellular composition in lesions was determined.

Results

Intralesion IL-6 in WF hosts was 99-fold lower than WW hosts (P = 0.03). Compared to WW host lesions, Cox-2 levels were decreased in WF [1.5-fold (= 0.02)] and FF [1.2-fold (P = 0.01)] host lesions, respectively, and intralesion VEGF expression was increased [1.8-fold; P = 0.02 (WF) and 1.5-fold; = 0.01 (FF)]. Lesions in FF hosts demonstrated reduced phosphohistone 3 expression (70%; = 0.03) compared to WW control hosts.

Conclusions

Systemic host 3-PUFA levels influence immune, angiogenic, and proliferative factors implicated in the early establishment of endometriosis.

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