A Potential Pathogenic Factor from Mycoplasma hominis is a TLR2-Dependent, Macrophage-Activating, P50-Related Adhesin
Article first published online: 17 JUN 2014
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
American Journal of Reproductive Immunology
Volume 72, Issue 3, pages 285–295, September 2014
How to Cite
A potential pathogenic factor from Mycoplasma hominis is a TLR2-dependent, macrophage-activating, P50-related adhesin. Am J Reprod Immunol 2014; 72: 285–295, , .
- Issue published online: 7 AUG 2014
- Article first published online: 17 JUN 2014
- Manuscript Accepted: 21 MAY 2014
- Manuscript Received: 17 MAR 2014
- Nora Eccles Treadwell Foundation
- Ministry of Education, Culture, Sports, Science and Technology. Grant Number: B2179178009
- Japan Society for the Promotion of Science. Grant Number: C23592692
- genital mycoplasmosis;
Mycoplasma hominis has been implicated in many inflammatory conditions of the human urogenital tract in particular amniotic infections that lead to fetal and neonatal disease and pre-term labor. The mechanisms responsible are poorly defined.
Method of Study
Biochemical and immunological methods were used to extract, purify, and characterize an inflammatory component present in M. hominis.
We isolated and purified to homogeneity a 40-kDa bioactive lipoprotein from M. hominis that was a potent TLR2-dependent, CD14-independent activator of the human THP-1 macrophage cell line. Homology searches of the N-terminal sequence revealed that 22 of the first 23 residues were identical to those seen for the phase-variable M. hominis p50 adhesin. The truncated P50t lipoprotein importantly retained its adhesive properties for human macrophages.
The unique adhesin/macrophage activator may play a key role in M. hominis infections by triggering an inflammatory cytokine cascade.