Novel Morphological Findings of Human Sperm Removal by Leukocytes in In Vivo and In Vitro Conditions: Preliminary Study

Authors

  • Malgorzata Piasecka,

    Corresponding author
    1. Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland
    • Correspondence

      Malgorzata Piasecka, Department of Histology and Developmental Biology, Pomeranian Medical University, Zolnierska 48, 71-210 Szczecin, Poland.

      E-mail: mpiasecka@ipartner.com.pl

      and

      Maciej Kurpisz, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.

      E-mail: kurpimac@man.poznan.pl

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    • Both authors equally contributed to this study.
  • Monika Fraczek,

    1. Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
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    • Both authors equally contributed to this study.
  • Dariusz Gaczarzewicz,

    1. Department of Animal Reproduction, Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland
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  • Kamil Gill,

    1. Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland
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  • Anna Szumala-Kakol,

    1. Unit of Microbiology, Hospital Medical College, Poznan, Poland
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  • Anna Kazienko,

    1. Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland
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  • Maria Laszczynska,

    1. Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland
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  • Stanislaw Lenart,

    1. Institute of Materials Engineering, West Pomeranian University of Technology, Szczecin, Poland
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  • Lothar Beutin,

    1. Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Berlin, Germany
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  • Maciej Kurpisz

    Corresponding author
    1. Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
    • Correspondence

      Malgorzata Piasecka, Department of Histology and Developmental Biology, Pomeranian Medical University, Zolnierska 48, 71-210 Szczecin, Poland.

      E-mail: mpiasecka@ipartner.com.pl

      and

      Maciej Kurpisz, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.

      E-mail: kurpimac@man.poznan.pl

    Search for more papers by this author

Abstract

Problem

Biological significance of increased number of leukocytes in ejaculate remains a subject of controversy. The aim of this research was to describe the morphological characteristics of the sperm elimination by leukocytes in in vivo and in vitro conditions using natural stimulator of the immune system—uropathogenic Escherichia coli, O75:HNT, isolated from semen.

Method of study

The study was performed on ejaculated spermatozoa from normozoospermic men with leukocytospermia (n = 10, in vivo experiment) or without leukocytospermia (n = 15, in vitro experiment). Morphological observations were performed using light and scanning electron microscopy.

Results

Sperm removal by active leukocytes mediated by traditional phagocytosis and generation of extracellular traps were observed in in vivo and in vitro experiments.

Conclusion

Our morphological data suggest that human germ cells activate leukocytes triggering both traditional phagocytosis and a novel trapping mechanism, followed by extensive sperm elimination.

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