Additional supporting information may be found in the online version of this article.


Figure S1: Renal allograft histology. (A) Termination graft histology of M45 (DCreg group; left) euthanized on Day 118 demonstrates more severe and widespread inflammation, interstitial fibrosis and tubular atrophy than M145 graft (control group; right) euthanized 66 days earlier on Day 52. Both show evidence of active tubulitis, as illustrated in the inserts. Low magnification images are 2× and the higher power inserts are 20×. (B) Graft biopsy from M113 (DCreg group) on Day 280 posttransplant (100 days after stopping immunosuppression) showing moderate to severe interstitial inflammation with active tubulitis. The insert (right) illustrates the area immediately above the (*) at higher magnification to illustrate the tubulitis and the focal peritubular capillaritis. Low magnification is 5× and the higher power insert is 60×.

Figure S2: DCreg infusion does not elicit circulating anti-donor alloAbs in renal-transplanted monkeys. Circulating anti-donor IgM and IgG alloAb levels were determined by flow cytometric analysis, as described in the Materials and Methods, before and at various times after transplant in control and DCreg-treated renal-allografted monkeys. Normal human serum served as a positive control. (A) Histograms are representative data of one recipient from each group. (B) MFI (mean florescence intensity) of IgM and IgG Abs binding to donor cells from all 6 transplant recipients in each group. Samples were collected before transplantation and 4–8 weeks after transplantation depending on the time of euthanasia. Bars indicate mean ± SD.

Figure S3: DCreg infusion does not affect the incidence of circulating Treg but reduces the Tmem:Treg ratio in transplanted monkeys. (A) Circulating levels of CD4+CD25+CD127loFoxp3+ Treg in control (n = 6) and DCreg-treated renal allografts recipients (n = 6), before and at various times post transplant. Data are means ± SD. (B) Reduced ratio of circulating CD4+CD95+ Tmem:Treg during prolonged graft survival in a DCreg-infused monkey (M113), followed by augmented ratio at the time of rejection.

Table S1: Propagation of DCreg from single leukapheresis products of 6 donor rhesus monkeys

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