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ajt12315-sm-0001-SupData.doc166KSupporting Information.
ajt12315-sm-0001-SupFig.pdf6K

Figure S1. Simvastatin treated DA rat macrophages do not directly affect on allogenic T cell activation. As the potential cellular effects of donor simvastatin treatment are carried along with the transplanted kidney, the possible primary targets of donor simvastatin treatment are kidney tissue cells and passenger leukocytes within the transplant. Here we wanted to study whether the simvastatin treatment of DA rats affect on allogenic T cell priming. (A) Quantitative real-time PCR analysis of isolated macrophages from DA rats 2 h after simvastatin 5 mg/kg (p.o) or vehicle treatment. (B) Major MHC-mismatched WF rat T cells were cultured for 5 days together with isolated macrophages of simvastatin or vehicle-treated DA rats. Anti-CD3 stimulation was used to assure the viability of the cells and native and syngenic cells were used as negative controls. ELISpot analysis of T cell INF-γ production at 5 days showed similar T cell activation for simvastatin or vehicle treatment groups. (A) The results were normalized to GAPDH molecule numbers and are given as a ratio to mRNA in vehicle-treated macrophages. (A, B) Mean values of duplicate samples were used for data interpretation. N = 3/group. *p < 0.05.

ajt12315-sm-0001-SupTab.doc28K

Table S1. Relative mRNA levels after simvastatin treatment in kidney allografts at 6 h and 5 days.

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