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Figure S1. Histological features of transplanted islet allografts. Representative macroscopic appearance of islet allografts from the long-term graft accepted and rejected animals is shown. Histological examination of the liver from a long-term graft accepted animal (M-#4) demonstrated the presence of islet allografts in the liver portal area (H&E), and no evidence of cellular infiltration, including that of CD3+, CD4+ or CD8+ cells, into or surrounding the islet allografts. In contrast, in the liver from the graft-rejected animal (M-#3), focal CD3+, CD4+ and CD8+ cellular infiltrates surrounding islet allografts were noted.

Figure S2. Cellular immune responses against third-party antigens. The frequency of directly (A) and indirectly (B) stimulated IFN-γ-secreting T cells against third-party antigens in the induction (left panels) and maintenance (right panels) treatment groups are shown. The results are presented as the fold change relative to the number before PITx. Both direct and indirect cellular responses against third-party antigens were elevated in all animals showing allograft rejection (I-#3, I-#4 and M-#3). In contrast, in recipient animals that accepted islets without allograft rejection (I-#5 and M-#4), these cellular responses were suppressed during the ASKP1240 treatment period but tended to recover after the discontinuation of ASKP1240 administration.

ajt12330-sm-0002-SupInfo-S1.doc73K

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