These authors contributed equally to this work.
Reversing Endogenous Alloreactive B Cell GC Responses With Anti-CD154 or CTLA-4Ig
Article first published online: 4 SEP 2013
© Copyright 2013 The American Society of Transplantation and the American Society of Transplant Surgeons
American Journal of Transplantation
Volume 13, Issue 9, pages 2280–2292, September 2013
How to Cite
Chen, J., Yin, H., Xu, J., Wang, Q., Edelblum, K. L., Sciammas, R. and Chong, A. S. (2013), Reversing Endogenous Alloreactive B Cell GC Responses With Anti-CD154 or CTLA-4Ig. American Journal of Transplantation, 13: 2280–2292. doi: 10.1111/ajt.12350
- Issue published online: 4 SEP 2013
- Article first published online: 4 SEP 2013
- Manuscript Accepted: 27 MAY 2013
- Manuscript Revised: 7 MAY 2013
- Manuscript Received: 28 JAN 2013
- NIAID/NIH. Grant Numbers: R03AI069284, R01AI083452
Additional Supporting Information may be found in the online version of this article at the publisher's web-site.
Figure S1: Splenocytes prepared from naïve C57BL/6 mice or on Day 14 after heart transplantation were stained with Kd-PE, Kd-FTIC and Kb-APC, or stained with Kd-PE, Kd-FITC and streptavidin(SAV)-APC. All data are the mean and SEM of 3–4 mice, and no significant differences in the percentage of Kb-APC- or SAV-APC-reactive Kd Tet-DP B cells are observed between the naïve and transplanted mice (p > 0.05).
Figure S2: Background staining with sera from naïve C57BL/6 mice on BALB/c targets. Sera from naïve mice or 7 days after BALB/c heart transplantation were incubated with BALB/c splenocytes, and then stained with anti-mouse IgG-FITC and anti-mouse IgM-PE. Control samples had no sera added. Data are presented as mean fluorescence intensity ± SEM (three mice each group).
Figure S3: IRF4 and CD138 staining of plasmablasts identifies a B220lo IRF4hi CD138+ plasmablast population. IRF4lo are activated B cells comprising the prememory/GC as well as the memory and GC subsets. CD138+B220hi B cells are likely to be activated B cells but not committed PCs.
Figure S4: Detailed gating strategy for Figures 5 and 6. After gating on lineage negative Dump−Kd Tet-DP population, ≥95% of the cells are B220+, so further B220 gating is not necessary. The Dump−Kd Tet-DP cells are then subdivided into IgDhi and IgDlo populations and their expression of GC (GL7+Fas+) or other markers (not shown) were assessed.
|ajt12350-sm-0002-SupTab-S1.docx||12K||Table S1: Germinal centers in lymph nodes from immunized and CTLA-4Ig treated mice.|
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