These authors contributed equally.
TCR Repertoire Analysis by Next Generation Sequencing Allows Complex Differential Diagnosis of T Cell–Related Pathology
Version of Record online: 10 SEP 2013
© Copyright 2013 The American Society of Transplantation and the American Society of Transplant Surgeons
American Journal of Transplantation
Volume 13, Issue 11, pages 2842–2854, November 2013
How to Cite
Dziubianau, M., Hecht, J., Kuchenbecker, L., Sattler, A., Stervbo, U., Rödelsperger, C., Nickel, P., Neumann, A. U., Robinson, P. N., Mundlos, S., Volk, H.-D., Thiel, A., Reinke, P. and Babel, N. (2013), TCR Repertoire Analysis by Next Generation Sequencing Allows Complex Differential Diagnosis of T Cell–Related Pathology. American Journal of Transplantation, 13: 2842–2854. doi: 10.1111/ajt.12431
- Issue online: 28 OCT 2013
- Version of Record online: 10 SEP 2013
- Manuscript Accepted: 8 JUL 2013
- Manuscript Revised: 19 JUN 2013
- Manuscript Received: 25 JAN 2013
- EFRE (Profit). Grant Number: 10145620
- BMBF (Primage)
Additional Supporting Information may be found in the online version of this article.
Figure S1: NGS reveals numerous clones with identical Vβ and Jβ genes, and CDR3 length but different amino acid sequences. CMV-specific (A) and CD3+ T cells (B and C) obtained from healthy individuals were sequenced using NGS-based approach. Clones with equal Vβ- and Jβ genes, and CDR3 length were analyzed. NGS delivers detailed information necessary for the correct clone discrimination. n = Absolute sequence counts.
Table S1: NGS allows analysis of extremely rare clonotypes.
Table S2: Clonotype kinetics of CMV/pp65-specific T cells.
Table S3: Sequences of primers used for amplification of the recombined TCRβ locus.
Table S4: Overview of clinical samples subjected to differential diagnosis of renal posttransplant dysfunction by means of NGS-based approach.
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