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Figure S1: (A) Blood glucose levels of STZ-ACI rats following (1) transplantation of 750 F344 islets into each of two prevascularized subcutaneous spaces at Day 0, (2) recovery of the left dorsal graft on Day 46 and (3) transplantation of 1500 F344 islets into the liver on Day 57. (B) Blood glucose levels of two STZ-Wistar rats following (1) transplantation of 3000 F344 islets in two prevascularized subcutaneous pockets and (2) removal of the left dorsal graft on Day 130.

Figure S2: Body weights of STZ-ACI rats transplanted with 3000 F344 islets into livers (A), nontreated subcutaneous (B) and prevascularized subcutaneous spaces (C). Double-slash (//) indicates recipients subjected to additional experiments to give some insight into the tolerance state (see Figure 4). (D) Body weights of STZ-ACI rats transplanted with 3000 Lewis islets in prevascularized subcutaneous tissue. Arrows indicate total graft recovery.

Figure S3: Immunofluorescence staining for (A-1) CD4 T cell, (B-1) macrophage (CD68), (C-1) granulocyte and (D) CD8 T cell of islet grafts retrieved from subcutaneous sites after 91 days allotransplantation in ACI rats. Normal ACI spleen tissues were used as positive controls the staining for (A2) CD4 T cell, (B2) macrophage and (C2) granulocytes staining. (E) Staining of spleen tissues without use of primary antibodies was used as negative controls. Islets, which were identified from paralleled section by H&E (F) and immunostaining for insulin, were denoted as “I” in the figures. All stained samples were prepared from a series of thin sections of the same islet graft. Secondary antibodies were fluorescently labeled with Alexa488 (green) or Alexa594 (red). Nucleus was counterstained with Hoechst (blue). Scale bar 50 µm.

Table S1: Allogeneic islet transplantation in diabetic rats in different experimental groups.

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