Both authors contributed equally.
Environmental bacteria and childhood asthma
Article first published online: 21 SEP 2012
© 2012 John Wiley & Sons A/S
Volume 67, Issue 12, pages 1565–1571, December 2012
How to Cite
Environmental bacteria and childhood asthma. Allergy2012; 67: 1565–1571., , , , , , , , .
Edited by: Reto Crameri
- Issue published online: 14 NOV 2012
- Article first published online: 21 SEP 2012
- Manuscript Accepted: 14 AUG 2012
- Deutsche Forschungsgemeinschaft
- European Union research grant. Grant Number: QLRT 1999-01391
- 16S rRNA;
- childhood asthma;
We have previously found an inverse association of bacterial diversity with childhood asthma. It remains unclear whether certain bacteria account for the protective effect.
The high variability of the bacterial 16S rRNA gene allows assessing diversity and specificity of bacterial communities by single-strand configuration polymorphism (SSCP). DNA was extracted from mattress dust samples of 489 school-age children from rural and suburban regions in Germany. A fragment of the bacteria-specific 16S rRNA gene was amplified by PCR, digested to single-strand DNA, and subjected to electrophoresis. The resulting band patterns reflect the underlying DNA sequences. The individual bands were tested for associations with asthma, hay fever, and atopy in quantitative and qualitative multivariable analyses. Significantly associated bands were isolated and sequenced. The sequences were compared to a database, and distinct bacteria were identified.
Seven of 76 independent bands were found to be inversely associated with asthma, atopic sensitization, and hay fever with odds ratios ranging from 0.17 to 0.73. The bands contained the sequences of Acinetobacter sp., Lactobacillus spp., Neisseria spp., Staphylococcus sciuri, Jeotgalicoccus sp., Corynebacterium spp., and others.
In a diverse microbial environment, certain bacteria may account for the protective effect on the development of asthma and atopy.