Edited by: Reto Crameri
Lipids are required for the development of Brazil nut allergy: the role of mouse and human iNKT cells
Article first published online: 9 NOV 2012
© 2012 John Wiley & Sons A/S
Volume 68, Issue 1, pages 74–83, January 2013
How to Cite
Lipids are required for the development of Brazil nut allergy: the role of mouse and human iNKT cells. Allergy 2012; DOI: 10.1111/all.12057., , , , , , , .
- Issue published online: 4 DEC 2012
- Article first published online: 9 NOV 2012
- Manuscript Accepted: 24 SEP 2012
- CNRS, Université René Descartes – Paris V
- Italian Ministry of Scientific Research and University. Grant Number: PRIN 2009RF7BY2
- FAPESP and CNPq
- NIH Tetramer
- anaphylactic antibodies;
- food allergy;
- NKT cells
Lipids are required for mice sensitization to Ber e 1, Brazil nut major allergen. Here, we characterized different lipid fractions extracted from Brazil nuts and the lipid-binding ability of Ber e 1. Further, we determined their in vivo ability to induce Ber-specific anaphylactic antibodies and the role of invariant natural killer T (iNKT) cells in this process.
Wild-type (WT) and iNKT cell-deficient mice were sensitized with Ber e 1 and specific lipid fractions, and anaphylactic antibodies were measured by enzyme-linked immunosorbent assay (ELISA) and passive cutaneous anaphylaxis (PCA). The lipid-binding characteristic of Ber e 1 (Ber) was established by using fluorescent probes and 15N-labeled NMR. In vitro production of IL-4 was determined in Ber/lipid C-stimulated mouse iNKT cells and human T-cell lines containing NKTs primed with CD1d+C1R transfectants by flow cytometry and ELISA, respectively.
Only one specific lipid fraction (lipid C), containing neutral and common phospholipids, induced Ber anaphylactic antibodies in mice. Ber e 1 has a lipid-binding site, and our results indicated an interaction between Ber e 1 and lipid C. iNKT-deficient mice produced lower levels of anaphylactic antibodies than WT mice. In vitro, Ber/lipid C-stimulated murine iNKT cells produced IL-4 but not IFN-gamma. Human T-cell lines derived from nut-allergic patients produced IL-4 to Ber/lipid C in a CD1d− and dose-dependent manner.
Lipid fraction C from Brazil nut presents an essential adjuvant activity to Ber e 1 sensitization, and iNKT cells play a critical role in the development of Brazil nut-allergic response.