Characteristics of anaphylaxis-inducing IgG immune complexes triggering murine passive systemic anaphylaxis

Authors

  • D. Jiao,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
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    • Delong Jiao and Yuan Liu contributed equally to this work.
  • Y. Liu,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
    2. Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China
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    • Delong Jiao and Yuan Liu contributed equally to this work.
  • X. Lu,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
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  • Q. Pan,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
    2. Institute of Nephrology, Guangdong Medical College, Zhanjiang, China
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  • J. Zheng,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
    2. Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China
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  • B. Liu,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
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  • Y. Liu,

    1. Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
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  • Y. Wang,

    1. Center for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China
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  • N. Fu

    Corresponding author
    • Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China
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  • Edited by: Hans-Uwe Simon

Correspondence

Ning Fu, Department of Immunology, Southern Medical University, 510515 Guangzhou, Guangdong, China.

Tel.: +86-20-61648221

Fax: +86-20-61648221

E-mail: zfzn53@yahoo.com.cn

Abstract

Background

With the broad and increasing application of therapeutic monoclonal antibodies (mAbs) in clinical settings, IgG-induced allergic reactions, including passive systemic anaphylaxis (PSA), have attracted significant attention. However, it is not clear which types of IgG mAb–antigen complexes or IgG aggregates formed by antigen binding can trigger PSA, as not all immune complexes (ICs) are capable of triggering PSA. Here, we characterise mAb–antigen complexes capable of inducing murine PSA to evaluate and predict which ICs are able to induce PSA.

Methods

Thirty-six combinatory reactions with eight antigens and 27 corresponding mAbs were used to trigger PSA, which was defined by rectal temperature. Sandwich ELISA, passive cutaneous anaphylaxis (PCA) induction and flow cytometry analysis of CD16/32 (FcγRIII/II) expression were used to characterise the ICs. The dynamic concentrations of antigen in the peripheral blood were measured by ELISA.

Results

Only 14 of the 36 ICs could trigger PSA and thus be termed anaphylaxis-inducing immune complexes (Ai-ICs). The Ai-ICs could be characterised by constructing sandwich ELISA, inducing PCA and down-regulating CD16/32 (FcγRIII/II) expression on blood neutrophils in vitro and in vivo. Additionally, the occurrence and severity of PSA was found to be associated with the instantaneous concentration of antigen in the peripheral blood in the presence of antibody.

Conclusions

Only Ai-ICs, not all ICs, could trigger IgG-mediated PSA, which could be characterised by the above simple methods. The occurrence and severity of PSA was associated with the instantaneous concentration of antigen in the peripheral blood in the presence of antibody.

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