all12143-sup-0001-Onlinesupplementary.docWord document43KData S1. Methods.
all12143-sup-0002-FigS1.epsimage/eps83KFigure S1. A–F: Kinetics of TNF-α, MMP-9 and inhibition of MMP-9 by anti-TNF-α over a time course of 21 h. Results are shown from three donors and normalised as % as release towards anti-IgE induced release at 21 h. Results given as ng/106 PBMCs for MMP-9 and pg/106 PBMCs for TNF-α. TNF-α release from PBMCs during 21 h with or without anti-IgE (Fig. 1A–B). MMP-9 release from PBMCs during 21 h with or without anti-IgE (Fig. 1C–D). MMP-9 release from PBMCs pre-incubated with or without anti-TNF-α before stimulation with anti-IgE for 21 h (Fig. 1E–F).
all12143-sup-0003-FigS2.epsimage/eps161KFigure S2. A–B. Cellular expression of TNF-α in pellets of purified basophils with or without anti-IgE stimulation (Fig. S2A). Additionally, basophil cell pellets were analysed with Western Blotting after 2 h stimulation with anti-IgE, and the molecular weight of detected TNF-α was found to be 17 kDa, indicating that basophils release TNF-α in a bio-active monomer form. Lysate from a THP-1 monocyte cell line indicates basal TNF-α expression as a positive control (Fig. S2B).
all12143-sup-0004-FigS3.epsimage/eps6597KFigure S3. Intracellular expression of TNF-α and IL-4 in cytospins of purified basophils with and without anti-IgE stimulation. Preliminary data obtained from two separate basophil donors. Results are shown following 2 h incubation ± anti-IgE. Bottom panel shows unstimulated basophils stained with May–Grünwald stain.

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