House dust mite-induced calcium signaling instigates epithelial barrier dysfunction and CCL20 production

Authors

  • S. Post,

    1. Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
    2. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
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  • M. C. Nawijn,

    1. Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
    2. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
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  • M. R. Jonker,

    1. Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
    2. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
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  • N. Kliphuis,

    1. Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
    2. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
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  • M. van den Berge,

    1. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    2. Department of Pulmonology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
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  • A. J. M. van Oosterhout,

    1. Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
    2. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
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  • I. H. Heijink

    Corresponding author
    1. GRIAC Research Institute, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    2. Department of Pulmonology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    • Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands
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  • Edited by: Hans-Uwe Simon

Correspondence

Irene H. Heijink, Department of Pathology and Medical Biology, Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen (UMCG), Hanzeplein 1, NL-9713GZ Groningen, the Netherlands.

Tel.: 31 (0)50 3610998

Fax: 31 (0)50 3619007

E-mail: h.i.heijink@umcg.nl

Abstract

Background

House dust mite (HDM) affects the immunological and physical barrier function of airway epithelium, leading to allergic sensitization, airway remodeling, and eosinophilic inflammation in mouse models, although the mechanisms are still largely unknown.

Objective

Given the implications for adenosine triphosphate (ATP)-dependent Ca2+ signaling in allergic sensitization in mice, we sought to determine the role of intracellular Ca2+ concentration ([Ca2+]i) in HDM-induced barrier dysfunction and pro-inflammatory activity of bronchial epithelium.

Methods

We investigated the effect of HDM on accumulation of [Ca2+]i levels, barrier function, and CCL20 release in human bronchial epithelial 16HBE cells and primary bronchial epithelial cells (PBECs) from healthy subjects and asthma patients. Involvement of ATP-dependent activation of purinergic receptors and downstream Ca2+ influx was studied, using the ATP hydrolyzing agent apyrase, the purinergic receptor agonist PPADS, the calcium chelator BAPTA-AM, and calpain inhibitors.

Results

Asthma PBECs were more susceptible to HDM-induced barrier dysfunction, CCL20 secretion, and Ca2+ influx than healthy PBECs. Furthermore, we show that the HDM-induced increase in CCL20 in PBECs and 16HBE cells and the HDM-induced barrier dysfunction in 16HBE cells are dependent on [Ca2+]i accumulation. Additionally, we demonstrate that [Ca2+]i accumulation is initiated partly through the activation of purinergic receptors, which contributes to HDM-induced epithelial barrier dysfunction by disruption of cell–cell contacts, but not CCL20 secretion.

Conclusion

Our data show for the first time that Ca2+ signaling plays a crucial role in barrier dysfunction and the pro-inflammatory response of bronchial epithelium upon HDM exposure and may thus have important implications for the development of allergic asthma.

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