Tolerogenic CX3CR1+ B cells suppress food allergy-induced intestinal inflammation in mice

Authors

  • Z.-Q. Liu,

    1. Department of Gastroenterology, Second Hospital, Zhengzhou University, Zhengzhou, China
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    • These authors share the first authorship.
  • Y. Wu,

    1. State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China
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    • These authors share the first authorship.
  • J.-P. Song,

    1. State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
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    • These authors share the first authorship.
  • X. Liu,

    1. State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China
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  • Z. Liu,

    Corresponding author
    1. State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China
    • Correspondence

      Dr. Ping-Chang Yang or Dr. Zhigang Liu, State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, 3688 Nanhai Ave, Shenzhen, Guangdong 518060, China.

      Tel.: +86 0755 86671907

      Fax: +86 0755 86671906

      E-mail: pcy2356@163.com

      or

      Dr. Peng-Yuan Zheng, Department of Gastroenterology, Second Hospital, Zhengzhou University, Zhengzhou, China.

      Tel.: +86 138 37128150

      Fax: +86 371 63934119

      E-mail: pengyuanzheng@163.com

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  • P.-Y. Zheng,

    Corresponding author
    1. Department of Gastroenterology, Second Hospital, Zhengzhou University, Zhengzhou, China
    • Correspondence

      Dr. Ping-Chang Yang or Dr. Zhigang Liu, State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, 3688 Nanhai Ave, Shenzhen, Guangdong 518060, China.

      Tel.: +86 0755 86671907

      Fax: +86 0755 86671906

      E-mail: pcy2356@163.com

      or

      Dr. Peng-Yuan Zheng, Department of Gastroenterology, Second Hospital, Zhengzhou University, Zhengzhou, China.

      Tel.: +86 138 37128150

      Fax: +86 371 63934119

      E-mail: pengyuanzheng@163.com

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  • P.-C. Yang

    Corresponding author
    1. State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China
    • Correspondence

      Dr. Ping-Chang Yang or Dr. Zhigang Liu, State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University and Institute of Allergy & Immunology, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, 3688 Nanhai Ave, Shenzhen, Guangdong 518060, China.

      Tel.: +86 0755 86671907

      Fax: +86 0755 86671906

      E-mail: pcy2356@163.com

      or

      Dr. Peng-Yuan Zheng, Department of Gastroenterology, Second Hospital, Zhengzhou University, Zhengzhou, China.

      Tel.: +86 138 37128150

      Fax: +86 371 63934119

      E-mail: pengyuanzheng@163.com

    Search for more papers by this author

  • Edited by: Angela Haczku
  • Correction added on 3 October 2013, after first online publication: order of author affiliations corrected.

Abstract

Background

B lymphocytes are an important cell population of the immune regulation; their role in the regulation of food allergy has not been fully understood yet.

Objective

This study aims to investigate the role of a subpopulation of tolerogenic B cells (TolBC) in the generation of regulatory T cells (Treg) and in the suppression of food allergy-induced intestinal inflammation in mice.

Methods

The intestinal mucosa-derived CD5+ CD19+ CX3CR1+ TolBCs were characterized by flow cytometry; a mouse model of intestinal T helper (Th)2 inflammation was established to assess the immune regulatory role of this subpopulation of TolBCs.

Results

A subpopulation of CD5+ CD19+ CX3CR1+ B cells was detected in the mouse intestinal mucosa. The cells also expressed transforming growth factor (TGF)-β and carried integrin alpha v beta 6 (αvβ6). Exposure to recombinant αvβ6 and anti-IgM antibody induced naive B cells to differentiate into the TGF-β-producing TolBCs. Coculturing this subpopulation of TolBCs with Th0 cells generated CD4+ CD25+ Foxp3+ Tregs. Adoptive transfer with the TolBCs markedly suppressed the food allergy-induced intestinal Th2 pattern inflammation in mice.

Conclusions

CD5+ CD19+ CX3CR1+ TolBCs are capable of inducing Tregs in the intestine and suppress food allergy-related Th2 pattern inflammation in mice.

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