Edited by: Reto Crameri
Identification of Maillard reaction products on peanut allergens that influence binding to the receptor for advanced glycation end products
Article first published online: 23 NOV 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd This article has been contributed to by US Government employees and their work is in the public domain in the USA
Volume 68, Issue 12, pages 1546–1554, December 2013
How to Cite
Identification of Maillard reaction products on peanut allergens that influence binding to the receptor for advanced glycation end products. Allergy 2013; 68: 1546–1554., , , , , , , , , , , , , , .
- Issue published online: 5 DEC 2013
- Article first published online: 23 NOV 2013
- Manuscript Accepted: 7 AUG 2013
- Agricultural Research Service
- U.S. Department of Agriculture
- advanced glycation end product;
- receptor for AGE
Recent immunological data demonstrated that dendritic cells preferentially recognize advanced glycation end product (AGE)-modified proteins, upregulate expression of the receptor for AGE (RAGE), and consequently bias the immune response toward allergy.
Peanut extract was characterized by mass spectrometry (MS) to elucidate the specific residues and specific AGE modifications found in raw and roasted peanuts and on rAra h 1 that was artificially glycated by incubation with glucose or xylose. The binding of the RAGE-V1C1 domain to peanut allergens was assessed by PAGE and Western analysis with anti-Ara h 1, 2, and 3 antibodies. IgE binding to rAra h 1 was also assessed using the same methods.
AGE modifications were found on Ara h 1 and Ara h 3 in both raw and roasted peanut extract. No AGE modifications were found on Ara h 2. Mass spectrometry and Western blot analysis demonstrated that RAGE binds selectively to Ara h 1 and Ara h 3 derived from peanut extract, whereas the analysis failed to demonstrate Ara h 2 binding to RAGE. rAra h 1 with no AGE modifications did not bind RAGE; however, after AGE modification with xylose, rAra h 1 bound to RAGE.
AGE modifications to Ara h 1 and Ara h 3 can be found in both raw and roasted peanuts. Receptor for AGE was demonstrated to selectively interact with AGE-modified rAra h 1. If sensitization to peanut allergens occurs in dendritic cells via RAGE interactions, these cells are likely interacting with modified Ara h 1 and Ara h 3, but not Ara h 2.