These authors contributed equally to this work.
Differences in exosome populations in human breast milk in relation to allergic sensitization and lifestyle
Article first published online: 15 JAN 2014
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 69, Issue 4, pages 463–471, April 2014
How to Cite
Differences in exosome populations in human breast milk in relation to allergic sensitization and lifestyle. Allergy 2014; 69: 463–471., , , , , , , .
Edited by: Bodo Niggemann
- Issue published online: 5 MAR 2014
- Article first published online: 15 JAN 2014
- Manuscript Accepted: 4 DEC 2013
- Swedish Research Council
- Cancer- and Allergy Foundation
- Centre for Allergy Research Karolinska Institutet
- Swedish Foundation for Health Care Sciences and Allergy Research
- Swedish Asthma and Allergy Association
- Samariten Foundation
- Hesselman Foundation
- Swedish Society for Medical Research
- Swedish Association for Allergology
- Milk Drop Foundation and the Karolinska Institutet
- allergic sensitization;
- anthroposophic lifestyle;
- breast milk;
Breast-feeding has many beneficial effects on the developing immune system of the newborn. Breast milk contains immunoregulatory factors, such as nano-sized vesicles named exosomes. This study aimed at characterizing breast milk exosomes from human early milk and mature milk and to investigate whether allergic sensitization and an anthroposophic lifestyle could influence the exosome profile.
Breast milk was collected from 22 mothers at day 3–8 and from 61 mothers at 2 months postpartum, all part of the ALADDIN birth cohort. Isolated exosomes were captured on anti-MHC-class II- or anti-CD63 beads and analyzed by flow cytometry. Exosomal phenotype was related to lifestyle and allergic sensitization of the mothers, and sensitization of the child at 2 years of age.
We found a higher content of exosomes in early milk compared with mature milk. Early milk exosomes were enriched in HLA-DR molecules and displayed significantly lower levels of HLA-ABC compared with those in mature milk. Phenotypically different subpopulations of exosomes were found in mature milk. Significantly lower levels of MUC1 were detected on CD63-enriched exosomes from sensitized mothers compared with nonsensitized. Furthermore, women with an anthroposophic lifestyle had significantly lower MUC1 expression on their HLA-DR-enriched milk exosomes and up-regulated levels of CD63 on CD63-enriched exosomes compared with nonanthroposophic mothers. Notably, mothers whose children developed sensitization had an increased amount of HLA-ABC on their milk exosomes enriched for CD63.
The phenotype of exosomes in breast milk varies with maternal sensitization and lifestyle, which might influence allergy development in the child.