These authors contributed equally to this work and are co-first authors for this study.
Icariin stimulates the proliferation of rat Sertoli cells in an ERK1/2-dependent manner in vitro
Version of Record online: 7 NOV 2012
© 2012 Blackwell Verlag GmbH
Volume 46, Issue 1, pages 9–16, February 2014
How to Cite
Nan, Y., Zhang, X., Yang, G., Xie, J., Lu, Z., Wang, W., Ni, X., Cao, X., Ma, J. and Wang, Z. (2014), Icariin stimulates the proliferation of rat Sertoli cells in an ERK1/2-dependent manner in vitro. Andrologia, 46: 9–16. doi: 10.1111/and.12035
- Issue online: 13 JAN 2014
- Version of Record online: 7 NOV 2012
- Manuscript Accepted: 4 SEP 2012
- National Natural Science Foundation of China. Grant Number: 3087326
- ERK ;
- Sertoli cell
Icariin (ICA), a major constituent of flavonoids from the Chinese medical herb Epimedium brevicornum Maxim, is found to be protective for male reproductive ability, with the underlying mechanism largely unknown. Our study here investigated the effects of ICA on Sertoli cells, which act as nurse cells for the germ cells developing. Icariin was found to stimulate Sertoli cell proliferation in a dose-dependent manner. Further study revealed that Icariin induced an obvious phosphorylation of ERK in Sertoli cells. Inhibition of activation of ERK by the ERK inhibitor U0126 nearly blocked the Icariin-induced proliferation of Sertoli cells. Taken together, our results suggest that Icariin promotes the proliferation of Sertoli cells in vitro by activating the ERK1/2 signal pathway, which might at least partially, explain the protective role of Icariin on male reproductive ability.