Effects of dietary astaxanthin on growth, antioxidant capacity and gene expression in Pacific white shrimp Litopenaeus vannamei

Authors

  • J. Zhang,

    1. Nutrition laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou, China
    2. Lab of Traditional Chinese Medicine and Marine Drugs, School of Life Science, Sun Yat-sen University, Guangzhou, China
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  • Y.-J. Liu,

    1. Nutrition laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou, China
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  • L.-X. Tian,

    1. Nutrition laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou, China
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  • H.-J. Yang,

    Corresponding author
    1. Nutrition laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou, China
    • Correspondence: H.-J. Yang, Nutrition Laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China. E-mail: edls@mail.sysu.edu.cn

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  • G.-Y. Liang,

    1. Nutrition laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou, China
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  • Y.-R. Yue,

    1. Nutrition laboratory, Institute of Aquatic Economical Animals, School of Life Science, Sun Yat-sen University, Guangzhou, China
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  • D.-H. Xu

    1. Lab of Traditional Chinese Medicine and Marine Drugs, School of Life Science, Sun Yat-sen University, Guangzhou, China
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Abstract

Pacific white shrimp Litopenaeus vannamei (1050 individuals with initial weight of 1.01 ± 0.001 g) were fed either control diet or one of six dietary astaxanthin (AX) concentration (25, 50, 75, 100, 125 and 150 mg kg−1) diets for 56 days in 35 tanks (30 shrimp per tank). After 56 days of culture, shrimp-fed AX125 and AX150 diets had higher (< 0.05) weight gain, specific growth rate, total antioxidant status and lower (< 0.05) superoxide dismutase (SOD), catalase (CAT) than shrimp fed control diet. After low dissolved oxygen stress for 1 h, survival rate of shrimp fed AX75, AX100, AX125 and AX150 diets was higher (< 0.05) than that of shrimp fed control diet. Hypoxia inducible factor-1α (HIF-1α), cytosolic manganese superoxide dismutase (cMnSOD) and CAT mRNA expression levels of shrimp fed seven diets were significantly down-regulated under hypoxia than under normoxia, but their expression levels were higher under hypoxia in shrimp fed AX-supplemented diets than in shrimp fed control diet. About 70-kDa heat-shock protein (Hsp70) mRNA expression level of shrimp fed seven diets was significantly up-regulated under hypoxia than under normoxia, but its expression level was lower under hypoxia in shrimp fed AX-supplemented diets than in shrimp fed control diet.

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