Differences between intestinal segments and soybean meal–induced changes in intestinal mucus composition of common carp Cyprinus carpio L.
Article first published online: 2 MAR 2013
© 2013 John Wiley & Sons Ltd
Volume 20, Issue 1, pages 12–24, February 2014
How to Cite
van der Marel, M., Pröpsting, M.J., Battermann, F., Jung-Schroers, V., Hübner, A., Rombout, J.H.W.M. and Steinhagen, D. (2014), Differences between intestinal segments and soybean meal–induced changes in intestinal mucus composition of common carp Cyprinus carpio L. Aquaculture Nutrition, 20: 12–24. doi: 10.1111/anu.12041
- Issue published online: 23 JAN 2014
- Article first published online: 2 MAR 2013
- Manuscript Accepted: 20 DEC 2012
- Manuscript Received: 13 SEP 2012
- DFG (Deutsche Forschungsgemeinschaft)
- antimicrobial peptides;
- intestinal segments;
The alimentary tract is a possible site where pathogens and toxins can enter. The alimentary tract is protected, amongst others, by mucus. In this study, tissue samples and crude mucus preparations from different parts of the intestinal tract of Cyprinus carpio (from intestinal bulb onto the hindgut) were examined using histological, histochemical and biochemical techniques. Furthermore, the response of the intestinal mucosal layer and intestinal mucus of C. carpio to acute soybean meal (SBM)-induced enteritis was investigated. In the present study, an indication for a different protein core of mucus high molecular weight glycoproteins (HMGs) for first and second segment could not be found. However, differences in mucus glycosylation could be found. Along the gut axis, the size of the major protein peaks were not similar, which can be caused by a different glycosylation. Also, differences in staining for the antimicrobial peptide beta-defensin 2 were found. Furthermore, changes in HMGs upon SBM diet were found similar to those found in inflammatory bowel disease (IBD) in humans. Initial changes include: changes in mucin composition, the presence of BD3 and of bacteria in internal organs. After the initial changes, all values measured returned back to the initial pre-SBM diet values.