The first author is C.-G. Tan, the co-first author is X.-Q. LI. These authors contribute equally to this work.
Effects of supplemental Azomite in diets on growth, immune function and disease resistance of white shrimp (Litopenaeus vannamei)
Version of Record online: 17 JAN 2014
© 2014 John Wiley & Sons Ltd
Volume 20, Issue 3, pages 324–331, June 2014
How to Cite
Tan, C.-G., Li, X.-Q., Leng, X.-J., Su, X.-G., Chen, L., Liu, B., Ma, F., Cai, X.-Q. and Guo, T. (2014), Effects of supplemental Azomite in diets on growth, immune function and disease resistance of white shrimp (Litopenaeus vannamei). Aquaculture Nutrition, 20: 324–331. doi: 10.1111/anu.12081
- Issue online: 13 MAY 2014
- Version of Record online: 17 JAN 2014
- Manuscript Accepted: 3 APR 2013
- Manuscript Received: 22 JUL 2012
- Shanghai Municipal Agricultural Commission. Grant Number: 2009-6-6
- Shanghai aquatic fishery key project. Grant Number: Y1101
- Lytone Co., Ltd.
- digestive enzymes;
- disease resistance;
- immune function;
- Litopenaeus vannamei
This study was conducted to evaluate effects of dietary Azomite, a natural mineral of volcanic ash, on growth performance, digestive enzyme activities, serum non-specific immunity and disease resistance of white shrimp (Litopenaeus vannamei). Graded levels of 0.0 g kg−1 (control group), 2.0, 4.0, 6.0 and 8.0 g kg−1 Azomite were supplemented in basal diet to feed shrimps. After 6 weeks feeding, weight gains of the 2.0 and 4.0 g kg−1 Azomite groups were significantly higher than that of the control group (P < 0.05). Compared with control group, the activities of stomach protease, hepatopancreas lipase, and serum alkaline phosphatase, lysozyme and phenoloxidase were significantly increased by the 4.0 g kg−1 Azomite addition (P < 0.05). When subjected to hypoxia stress, the lethal times of the first shrimp and all shrimps were both delayed by the addition of 2.0–6.0 g kg−1 Azomite to diets, compared with the lethal time of control group. At the 96th hour after the injection of bacterium (Vibrio alginolyticus), the accumulated mortality of 4.0 g kg−1 Azomite addition group was 34.6% lower than that of control group (P < 0.05). The results showed that dietary levels of 2.0 and 4.0 g kg−1 Azomite can improve the growth performance, digestive enzyme activities, serum non-specific immune function and disease resistance of white shrimp.